"Con A" Related Products

Con A Agarose

Cat.no: Con A-001A
Product Overview: Con A agarose is an affinity medium with concanavalin A (Con A) coupled to agarose 4B by the cyanogen bromide method, which is a?chromatography?medium?for?separation?and?purification?of?glycoproteins,?polysaccharides,?and?glycolipids.
Characteristics: ConA binds specifically α-D-mannosyl and α-D-glucosyl residues in terminal position of ramified structures from B-Glycans. It has 4 binding sites, corresponding to the 4 sub-units. The molecular weight is 104-112KDa and the isoelectric point (pI) is in the range of 4.5-5.5. The binding sugar requires the presence of C-3, C-4 and C-5 hydroxyl groups for reaction with Con A. Con A coupled to agarose is routinely used for separation and purification of glycoproteins, polysaccharides and glycolipids.
Size: 5 mL, 100 mL
Matrix: 4% agarose
Functional group: Con A
Average particle size: 90μm
Ligand: Con A
Ligand concentration: 10?to?15?mg?Con?A/ml?medium
Dynamic binding capacity: 20-45?mg?porcine?thyroglobulin/ml?medium
Recommended flow rate: 75 cm/h
Recommended column height: 5‐20cm
Chemical stability: Stable?in?all?commonly?used?aqueous?buffers.?Chelating?agents?such?as?EDTA,?8?M?urea,?or?solutions?having?pH?below?3?should?be?avoided?as?these?conditions?result?in?removal?of?manganese?from?the?lectin?with?loss?of?activity as a result.
pH working range: 4-9
Warning: Avoid using Chelating agents such as EDTA, 8 M urea or solutions having a pH below 3 should be avoided as these conditions results in removal of manganese from the lectin with loss of activity as a result.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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