"Chelate" Related Products

Chelate Cellulose resin

Cat.no: Chelate-001C
Product Overview: Chelate Cellulose resin is designed for immobilized metal chelate affinity chromatography of proteins and peptides. This media comprised of spherical cellulose beads to which iminodiacetic acid (IDA) has been immobilized.
Characteristics: Chelate Cellulose resin is based on porous, spherical, crosslinked cellulose beads and has been specifically optimized for metal affinity chromatography. It offers high capacity and good flow rate properties in large industrial columns. The cellulose backbone offers very low non-specific adsorption and the covalent bonding of iminodiacetic acid ensures ligand stability.
Matrix: cellulose
Dynamic binding capacity: 22 - 30 μM/mL Zn2+; 35 - 45 μM/mL Cu2+
Recommended flow rate: 20 – 150 cm/h
Chemical stability: Compatible with most salts, detergents and chaotropic agents. The operating pH range is 3 – 12. Autoclavable in suspension at neutral pH for 30 minutes at 121°C.
Physical stability: Compatible with most salts, detergents and chaotropic agents. The operating pH range is 3 – 12. Autoclavable in suspension at neutral pH for 30 minutes at 121°C.
pH working range: 2-12
Shelf life: 5 years from date of manufacture
Warning: The sample should not contain chelating substances such as EDTA or citric acid.
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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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