Heparin Agarose High Performance

• Cat.No.: Heparin-001A
• Product Overview: Heparin HP columns are prepacked with Heparin Agarose High Performance for high-resolution purification of proteins with affnity for heparin such as DNA-binding proteins, coagulation factors, lipoproteins, and protein synthesis factors.

Specification

• Cat.no: Heparin-001A
• Characteristics: Heparin is coupled to the Agarose High Performance base matrix via the N-hydroxysuccinamide coupling method, to give high capacity, performance, and low leakage levels. HiTrap Heparin HP is delivered in 0.2 M NaCl with 20% ethanol as bacteriostatic agent. It can operate as an affinity ligand with affinity for a wide range of biomolecules, including coagulation factors and other plasma proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors. Heparin also functions as a high capacity cation exchanger, due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases.
• Size: 1 mL, 5 mL, 25mL
• Matrix: Highly cross-linked agarose, 6%
• Average particle size: 34 μm
• Ligand: Porcine heparin
• Ligand concentration: 10 mg heparin/ml medium
• Dynamic binding capacity: 3 mg antithrombin III (bovine)/ml medium
• Recommended flow rate: 0.2 to 1 ml/min and 1.5 ml/min for 1 ml and 5 ml column respectively
• Chemical stability: All commonly used buffers
• pH working range: 5-10
• pH CIP range: 5-10