"Heparin" Related Products

Heparin Agarose High Performance

Cat.no: Heparin-001A
Product Overview: Heparin HP columns are prepacked with Heparin Agarose High Performance for high-resolution purification of proteins with affnity for heparin such as DNA-binding proteins, coagulation factors, lipoproteins, and protein synthesis factors.
Characteristics: Heparin is coupled to the Agarose High Performance base matrix via the N-hydroxysuccinamide coupling method, to give high capacity, performance, and low leakage levels. HiTrap Heparin HP is delivered in 0.2 M NaCl with 20% ethanol as bacteriostatic agent. It can operate as an affinity ligand with affinity for a wide range of biomolecules, including coagulation factors and other plasma proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors. Heparin also functions as a high capacity cation exchanger, due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases.
Size: 1 mL, 5 mL, 25mL
Matrix: Highly cross-linked agarose, 6%
Average particle size: 34 μm
Ligand: Porcine heparin
Ligand concentration: 10 mg heparin/ml medium
Dynamic binding capacity: 3 mg antithrombin III (bovine)/ml medium
Recommended flow rate: 0.2 to 1 ml/min and 1.5 ml/min for 1 ml and 5 ml column respectively
Chemical stability: All commonly used buffers
pH working range: 5-10
pH CIP range: 5-10
Products Types

◆ Native Protein

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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