Phenyl Agarose Fast Flow Phenyl FF-001A

Phenyl Agarose Fast Flow

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Phenyl Agarose Fast Flow

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Cat.no : Phenyl FF-001A
Product Overview : Phenyl FF are prepacked Phenyl agarose 6 Fast Flow columns for hydrophobic interaction chromatography (HIC). The columns are used for small-scale protein purification as well as for screening of binding and elutions conditions.
Characteristics : Phenyl agarose Fast Flow is a well-established, standard aromatic HIC medium for capture and intermediate purification requiring low to medium hydrophobicity which is based on a 90 μm matrix, making it idealfor initial and intermediate step purifications requiring a matrix with medium to high hydrophobicity. The availability of two degrees of substitution grades increases the possibility of finding the best selectivity and capacity for a given application.
Form : Slurry in 20% Ethanol
Size : 5 mL
Column : HiTrap
Matrix : 6% crosslinked agarose with spherical beads
Average particle size : 90 μm
Ligand : Phenyl
Ligand concentration : 25-40 μmol/ml medium
Chemical stability : Good chemical stability allows the use of the prepacked columns with most commonly used buffers and organic solvents, like for example 1 M NaOH, 70% ethanol, 30% isopropanol, 6 M GuHCl and 8 M urea. The compatibility with other solvents have been verified by conducting stability test for some of the media with 1 M acetic acid, 3 M (NH4) SO4 and 1 M HCl.
pH working range : 3-13
pH CIP range : 2-14
Temperature Stability : 4‐40 °C
Shelf life : minimum 5 years

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