"Phenyl" Related Products

Phenyl Agarose Fast Flow

Cat.no: Phenyl FF-001A
Product Overview: Phenyl FF are prepacked Phenyl agarose 6 Fast Flow columns for hydrophobic interaction chromatography (HIC). The columns are used for small-scale protein purification as well as for screening of binding and elutions conditions.
Characteristics: Phenyl agarose Fast Flow is a well-established, standard aromatic HIC medium for capture and intermediate purification requiring low to medium hydrophobicity which is based on a 90 μm matrix, making it idealfor initial and intermediate step purifications requiring a matrix with medium to high hydrophobicity. The availability of two degrees of substitution grades increases the possibility of finding the best selectivity and capacity for a given application.
Form: Slurry in 20% Ethanol
Size: 5 mL
Column: HiTrap
Matrix: 6% crosslinked agarose with spherical beads
Average particle size: 90 μm
Ligand: Phenyl
Ligand concentration: 25-40 μmol/ml medium
Chemical stability: Good chemical stability allows the use of the prepacked columns with most commonly used buffers and organic solvents, like for example 1 M NaOH, 70% ethanol, 30% isopropanol, 6 M GuHCl and 8 M urea. The compatibility with other solvents have been verified by conducting stability test for some of the media with 1 M acetic acid, 3 M (NH4) SO4 and 1 M HCl.
pH working range: 3-13
pH CIP range: 2-14
Temperature Stability: 4‐40 °C
Shelf life: minimum 5 years
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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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