||Dextran is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Dextran G-75 is well established gel filtration medium for desalting and buffer exchange of large biomolecules >80 000 molecular weight.
||Dextran G-75 is for larger molecules. Its features are as follows:1. Quickly desalts, removes contaminants and transfers to a new buffer in a single step.2. Suitable for buffer exchange and cleanup of large biomolecules, >80 000 molecular weight3. Highest exclusion limit, 80 000, in the Dextran G-range Classic gel filtration medium.
|Average particle size:
||particle wet: 90 μm-280 μmparticle dry: 40 μm-120 μm
|Recommended flow rate:
||Dextran is stable in all commonly used aqueous buffers and additives such as ionic and non-ionic detergents, denaturing agents (8 M urea or 6 M guanidine hydrochloride). The media are stable in short chain alcohols such as ethanol, methanol and propanol, but concentrations above 25% should not normally be used. Note that Dextran shrinks in alcohol solutions.
|pH working range:
||Avoid changes in temperature which may cause air bubbles in the packing.