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Recombinant Human COCH cell lysate

Cat.No. : COCH-2373HCL
Product Overview : Human COCH / Cochlin ( isoform short ) derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : COCH coagulation factor C homolog, cochlin (Limulus polyphemus) [ Homo sapiens ]
Official Symbol : COCH
Synonyms : COCH; coagulation factor C homolog, cochlin (Limulus polyphemus); coagulation factor C (Limulus polyphemus homolog); cochlin , DFNA9, DFNA31; cochlin; COCH 5B2; DFNA9; COCH5B2; COCH-5B2;
Gene ID : 1690
mRNA Refseq : NM_001135058
Protein Refseq : NP_001128530
MIM : 603196
UniProt ID : O43405
Chromosome Location : 14q11.2-q13

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How do genetic mutations in the COCH gene affect hearing and balance? 03/03/2023

Mutations in COCH can lead to impaired cochlear function, resulting in hearing and balance disorders.

How does COCH contribute to the maintenance of cochlear structure and function? 01/06/2023

It plays a key role in the structural integrity and mechanical properties of the cochlear extracellular matrix.

How does COCH interact with other proteins in the cochlea to support hearing? 09/28/2022

COCH works with other cochlear proteins to maintain the mechanics and signal transduction necessary for hearing.

What potential therapeutic approaches could target COCH for treating hearing loss? 06/04/2022

Therapeutic strategies targeting COCH could offer new treatments for genetic and age-related hearing loss.

What is the impact of COCH dysfunction in age-related hearing loss? 07/08/2021

COCH dysfunction is associated with the progression of age-related hearing loss, affecting cochlear homeostasis.

What is the primary function of COCH in the inner ear and hearing process? 01/16/2019

COCH is essential for maintaining the structure and function of the cochlea, crucial for hearing.

What role does COCH play in the development and progression of hearing loss disorders? 04/07/2018

COCH dysfunctions are closely linked to various types of hearing loss, including sensorineural hearing loss.

Customer Reviews (3)

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Reviews
07/28/2020

    Key contributor to our research discoveries.

    05/24/2018

      Swift and accurate service, a research necessity.

      11/03/2017

        Invaluable support for our protein experiments.

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