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| Cat.No.: | Brain-8H |
| Species: | Human |
| Form: | The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
| Concentration: | 2.0 mg/ml |
| Tissue Type: | Brain |
| Preparation method: | Brain tumor lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. |
| Safety Testing: | This material has been tested by accepted techniques and has been found to be negative for HBsAg, HIV 1/2, and HCV. Additional patient information may be available upon request. |
| Diagnosis: | Tumor |
| Expiration Date: | One year from date of receipt |
| Size: | 0.1 mg, 0.5 mg |
| Storage Instruction: | This lysate should be aliquoted in working amounts and may be stored for up to one month at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. For longer term storage, freeze working aliquots at -70°C. |
| Applications: | This lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel. |
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