Human Brain Tumor Tissue Lysate Brain-8H

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Human Brain Tumor Tissue Lysate

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Cat.No. :	Brain-8H
Species :	Human
Form :	The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.
Concentration :	2.0 mg/ml
Tissue Type :	Brain
Preparation method :	Brain tumor lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation.
Safety Testing :	This material has been tested by accepted techniques and has been found to be negative for HBsAg, HIV 1/2, and HCV. Additional patient information may be available upon request.
Diagnosis :	Tumor
Expiration Date :	One year from date of receipt
Size :	0.1 mg, 0.5 mg
Storage Instruction :	This lysate should be aliquoted in working amounts and may be stored for up to one month at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. For longer term storage, freeze working aliquots at -70°C.
Applications :	This lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.