| Species : |
Human |
| Tag : |
Non |
| Form : |
The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
| Concentration : |
2.0 mg/ml |
| Tissue Type : |
Brain |
| Preparation method : |
Brain tumor lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. |
| Safety Testing : |
This material has been tested by accepted techniques and has been found to be negative for HBsAg, HIV 1/2, and HCV. Additional patient information may be available upon request. |
| Diagnosis : |
Tumor |
| Expiration Date : |
One year from date of receipt |
| Size : |
0.1 mg, 0.5 mg |
| Storage Instruction : |
This lysate should be aliquoted in working amounts and may be stored for up to one month at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. For longer term storage, freeze working aliquots at -70°C. |
| Applications : |
This lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel. |
