Recombinant C. difficile toxB, His-tagged toxB-12C

Recombinant C. difficile toxB, His-tagged


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Recombinant C. difficile toxB, His-tagged

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Cat.No. : toxB-12C
Product Overview : Recombinant C. difficile toxB (Met1-Leu543), fused with a C-terminal 6-His tag, was expressed in E. coli.
Description : Clostridium difficile toxin B is a toxin produced by the bacteria Clostridium difficile. C. difficile produces two major kinds of toxins that are very potent and lethal; an enterotoxin (Toxin A) and a cytotoxin (Toxin B, this protein).
Source : E. coli
Species : C. difficile
Tag : His
Predicted N Terminal : Ser2
Form : Supplied as a 0.2 µm filtered solution in Tris and NaCl.
Bio-activity : Measured by its ability to hydrolyze UDP-Glucose.
Molecular Mass : Predicted Molecular Mass: 64 kDaSDS-PAGE: 60-65 kDa, reducing conditions
Endotoxin : < 1.0 EU per 1 μg of the protein by the LAL method.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
Storage : Avoid repeated freeze-thaw cycles.6 months from date of receipt, -20 to -70 °C as supplied.3 months, -20 to -70 °C under sterile conditions after opening.

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