"UBE2A" Related Products

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Recombinant Human UBE2A 293 Cell Lysate

Cat.No.: UBE2A-594HCL
Description: Antigen standard for ubiquitin-conjugating enzyme E2A (RAD6 homolog) (UBE2A), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: UBE2A ubiquitin-conjugating enzyme E2A [ Homo sapiens ]
Official Symbol: UBE2A
Synonyms: UBE2A; ubiquitin-conjugating enzyme E2A; ubiquitin conjugating enzyme E2A (RAD6 homolog); ubiquitin-conjugating enzyme E2 A; HHR6A; RAD6A; UBC2; HR6A; RAD6 homolog A; ubiquitin-protein ligase A; ubiquitin carrier protein A; ubiquitin-conjugating enzyme E2A (RAD6 homolog); MRXSN; MRXS30;
Gene ID: 7319
mRNA Refseq: NM_181762
Protein Refseq: ?NP_861427
MIM: 312180
UniProt ID: P49459
Chromosome Location: Xq24
Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem; Ubiquitin mediated proteolysis, organism-specific biosystem;

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