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Recombinant Human AKT1, His-tagged

Cat. No.: AKT1-14H
Product Overview: Recombinant Human AKT1 produced inSF9is a glycosylated, polypeptide chain containing 481 amino acids and having a molecular mass of 60 KD. This protein is the full-length form of the protein with a N-terminal His-tag. AKT1 is purified by proprietary chromatographic techniques.
Description: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
Source: Baculovirus, SF9 insect cells.
Unit Definition: 250 units/mg. One unit of Akt1 activity is equal to 1 nanomole of phosphate transferred to crosstide substrate per minute at 30°C with a final ATP concentration of 100µM. The optimal concentration should be determined for each specific application. Please note: Kinase activity may vary depending on the substrate and reaction conditions.
Physical Appearance: Sterile Filtered clear solution.
Purity: Greater than 95% as determined by SDS-PAGE. Mass spectrometry of tryptic digest products for identity determination.
Formulation: AKT1 (0.1mg/ml) is supplied in 50mM Tris pH 7.5, 0.15M NaCl, 0.27M sucrose,10mM beta-mercaptoethanol, 1mM EGTA. Carrier-free.
Storage: Store at 4°C if entire vial will be used within 1-2 weeks. Store, frozen at -20°C- -80°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Gene Name: AKT1 v-akt murine thymoma viral oncogene homolog 1 [ Homo sapiens ]
Synonyms: v-akt murine thymoma viral oncogene homolog 1; AKT; PKB; RAC; PRKBA; MGC99656; PKB-ALPHA; RAC-ALPHA; AKT1; AKT1 kinase; protein kinase B; rac protein kinase alpha; RAC-alpha serine/threonine-protein kinase; C-AKT; EC 2.7.11.1; RAC-PK-alpha; Protein kinase B
Gene ID: 207
mRNA Refseq: NM_001014431
Protein Refseq: NP_001014431
MIM: 164730
UniProt ID: P31749
Chromosome Location: 14q32.32; 14q32.32
Pathway: Acute myeloid leukemia; Adipocytokine signaling pathway; Apoptosis; B cell receptor signaling pathway; Chemokine signaling pathway; Chronic myeloid leukemia; Colorectal cancer; Endometrial cancer; ErbB signaling pathway; Fc epsilon RI signaling pathway; Fc gamma R-mediated phagocytosis; Focal adhesion; Glioma; Insulin signaling pathway; Jak-STAT signaling pathway; MAPK signaling pathway; Melanoma; Neurotrophin signaling pathway; Non-small cell lung cancer; Pancreatic cancer; Pathways in cancer; Prostate cancer; Renal cell carcinoma; Small cell lung cancer; T cell receptor signaling pathway; Tight junction; Toll-like receptor signaling pathway; VEGF signaling pathway; mTOR signaling pathway; Regulation of beta-cell development; Apoptosis; Hemostasis; Metabolism of nitric oxide; Signaling by GPCR; Signaling in Immune system; Signalling by NGF
Function: phosphatidylinositol-3,4,5-trisphosphate binding; phosphatidylinositol-3,4-bisphosphate binding; protein serine/threonine kinase activity; nitric-oxide synthase regulator activity; identical protein binding; transferase activity; enzyme binding; ATP binding

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