Recombinant Human AKT1 Protein, His-tagged

• Cat.No.: AKT1-14H
• Product Overview: Recombinant human AKT1 (1-480aa) protein with His tag was expressed in baculovirus.

Specification

• Description: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.
• Source: Insect cells
• Species: Human
• Tag: His
• Form: Liquid
• Molecular Mass: 56.7 kDa
• Endotoxin: < 1.0 EU per 1 μg of protein (determined by LAL method)
• Purity: > 90% by SDS-PAGE.
• Notes: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/mL (determined by absorbance at 280 nm)
• Storage Buffer: In phosphate buffered saline (pH7.4), 20% glycerol.

Gene Information

• Gene Name: AKT1 AKT serine/threonine kinase 1 [ Homo sapiens (human) ]
• Official Symbol: AKT1
• Synonyms: AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA; AKT1; AKT serine/threonine kinase 1; RAC-alpha serine/threonine-protein kinase; AKT1m; PKB alpha; RAC-PK-alpha; protein kinase B alpha; proto-oncogene c-Akt; rac protein kinase alpha; serine-threonine protein kinase; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1; EC 2.7.11.1
• Gene ID: 207
• mRNA Refseq: NM_001014432
• Protein Refseq: NP_001014432
• MIM: 164730
• UniProt ID: P31749