"PHLPP1" Related Products

Recombinant Human PHLPP1 293 Cell Lysate

Cat.No.: PHLPP1-3218HCL
Description: Antigen standard for PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PHLPP1 PH domain and leucine rich repeat protein phosphatase 1 [ Homo sapiens ]
Official Symbol: PHLPP1
Synonyms: PHLPP1; PH domain and leucine rich repeat protein phosphatase 1; PH domain and leucine rich repeat protein phosphatase , PHLPP, pleckstrin homology domain containing, family E (with leucine rich repeats) member 1 , PLEKHE1; PH domain leucine-rich repeat-containing protein phosphatase 1; KIAA0606; SCOP; SCN circadian oscillatory protein; PH domain-containing family E member 1; suprachiasmatic nucleus circadian oscillatory protein; pleckstrin homology domain containing, family E (with leucine rich repeats) member 1; PHLPP; PLEKHE1; MGC161555;
Gene ID: 23239
mRNA Refseq: NM_194449
Protein Refseq: NP_919431
MIM: 609396
UniProt ID: O60346
Chromosome Location: 18q21.32
Pathway: Adaptive Immune System, organism-specific biosystem; Disease, organism-specific biosystem; Downstream Signaling Events Of B Cell Receptor (BCR), organism-specific biosystem; Downstream signal transduction, organism-specific biosystem; Downstream signaling of activated FGFR, organism-specific biosystem; GAB1 signalosome, organism-specific biosystem; Immune System, organism-specific biosystem;
Function: hydrolase activity; metal ion binding; protein serine/threonine phosphatase activity;

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.