"ATP11C" Related Products

Recombinant Human ATP11C 293 Cell Lysate

Cat.No.: ATP11C-8615HCL
Description: Antigen standard for ATPase, class VI, type 11C (ATP11C), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ATP11C ATPase, class VI, type 11C [ Homo sapiens ]
Official Symbol: ATP11C
Synonyms: ATP11C; ATPase, class VI, type 11C; ATPase, Class VI, type 11C; probable phospholipid-transporting ATPase IG; ATPIG; ATPIQ; phospholipid-transporting ATPase IG;
Gene ID: 286410
mRNA Refseq: NM_001010986
Protein Refseq: NP_001010986
MIM: 300516
UniProt ID: Q8NB49
Chromosome Location: Xq27.1
Pathway: Ion channel transport, organism-specific biosystem; Ion transport by P-type ATPases, organism-specific biosystem; Transmembrane transport of small molecules, organism-specific biosystem;
Function: ATP binding; ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism; hydrolase activity; hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances; magnesium ion binding; nucleotide binding; phospholipid-translocating ATPase activity; protein binding;

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