Recombinant Human ATF1 293 Cell Lysate

• Cat.No.: ATF1-8632HCL

Specification

• Description: Antigen standard for activating transcription factor 1 (ATF1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Publication:
  CCR5AS lncRNA variation differentially regulates CCR5, influencing HIV disease outcome (2019)

Gene Information

• Gene Name: ATF1 activating transcription factor 1 [ Homo sapiens ]
• Official Symbol: ATF1
• Synonyms: ATF1; activating transcription factor 1; cyclic AMP-dependent transcription factor ATF-1; TREB36; cAMP-dependent transcription factor ATF-1; EWS-ATF1; FUS/ATF-1
• Gene ID: 466
• mRNA Refseq: NM_005171
• Protein Refseq: NP_005162
• MIM: 123803
• UniProt ID: P18846
• Chromosome Location: 12q13
• Pathway: Activated TLR4 signalling, organism-specific biosystem; CREB phosphorylation, organism-specific biosystem; EGFR1 Signaling Pathway, organism-specific biosystem; ErbB1 downstream signaling, organism-specific biosystem; HTLV-I infection, organism-specific biosystem; HTLV-I infection, conserved biosystem; IL-3 Signaling Pathway, organism-specific biosystem
• Function: protein complex binding; protein heterodimerization activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific distal enhancer binding RNA polymerase II transcription factor activity