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Recombinant Human ARL5A 293 Cell Lysate

Cat.No. : ARL5A-8710HCL
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  • Gene Information
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Description : Antigen standard for ADP-ribosylation factor-like 5A (ARL5A), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ARL5A ADP-ribosylation factor-like 5A [ Homo sapiens ]
Official Symbol : ARL5A
Synonyms : ARL5A; ADP-ribosylation factor-like 5A; ADP ribosylation factor like 5 , ARL5; ADP-ribosylation factor-like protein 5A; ADP-ribosylation factor-like 5; ADP-ribosylation factor-like protein 5; ARL5; ARFLP5;
Gene ID : 26225
mRNA Refseq : NM_001037174
Protein Refseq : NP_001032251
MIM : 608960
UniProt ID : Q9Y689
Chromosome Location : 2q23.3
Function : GTP binding; nucleotide binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (12)

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Is there any evidence of ARL5A involvement in signal transduction pathways? 01/17/2022

While ARL5A is primarily associated with cellular trafficking processes, it can indirectly influence signal transduction pathways by modulating the function of primary cilia. Primary cilia play a critical role in sensing extracellular signals and transmitting them to intracellular signaling pathways, and ARL5A contributes to ciliary function.

Can ARL5A be targeted for therapeutic purposes? 09/28/2021

Currently, there are no known therapeutic interventions specifically targeting ARL5A. However, the understanding of its role in diseases, particularly in ciliary dysfunction disorders, may pave the way for future therapeutic strategies aimed at modulating ARL5A activity or related pathways.

Is the expression of ARL5A protein regulated during cellular development? 05/17/2021

Yes, studies suggest that the expression of ARL5A can be regulated during cellular development and differentiation. Its levels may vary in different tissues and cell types, indicating potential roles in developmental processes.

Are there any known post-translational modifications of ARL5A protein? 10/06/2020

Yes, ARL5A can undergo post-translational modifications, including prenylation. Prenylation involves the addition of a lipid group (such as a farnesyl or geranylgeranyl group) to the C-terminus of the protein, which aids in its membrane targeting and function.

Are there any known interacting proteins or binding partners of ARL5A? 08/20/2020

Yes, several proteins have been identified as interacting partners of ARL5A. Some examples include ARF-like protein 6 (ARL6), ARL6-interacting protein (ARL6IP1), and Arfaptin-2 (ARFIP2), which interact with ARL5A to regulate vesicle trafficking and ciliary function.

Are there any known animal models for studying ARL5A function or disease associations? 02/22/2020

Animal models with mutations or deletions in the Arl5a gene have been generated, primarily in mice, to study its function and possible disease associations. These models can provide valuable insights into the role of ARL5A in cellular processes and disease pathogenesis.

Can dysregulation of ARL5A expression or function contribute to neurodegenerative disorders? 08/11/2019

Although ARL5A's specific involvement in neurodegenerative disorders is not well-established, disruption of cellular trafficking and protein sorting, which are regulated by ARL5A, can contribute to the pathology of various neurodegenerative diseases.

In what cellular processes is ARL5A involved? 02/26/2019

ARL5A is involved in various cellular processes, including the formation and maintenance of primary cilia, a cellular organelle involved in signal transduction. It also plays a role in intracellular trafficking, specifically in the regulation of protein sorting, vesicle formation, and protein transport between compartments.

Are there any disease-specific mutations identified in the ARL5A gene? 10/05/2018

Yes, mutations in the ARL5A gene have been identified in individuals with Bardet-Biedl syndrome, suggesting a disease-specific involvement of ARL5A. These mutations can disrupt the normal function of ARL5A and impair cellular processes dependent on ciliary function.

How is the activity of ARL5A regulated? 01/03/2018

The activity of ARL5A is regulated by the binding and hydrolysis of GTP (guanosine triphosphate). When bound to GTP, ARL5A is in its active state, promoting cellular processes. It hydrolyzes GTP to GDP (guanosine diphosphate), leading to inactivation and release from its target proteins.

What are the functional consequences of ARL5A knockdown or knockout? 04/24/2016

Studies involving ARL5A knockdown or knockout have demonstrated several functional consequences. These include impaired ciliogenesis, disrupted vesicular trafficking, altered protein sorting, and abnormal cellular signaling. The absence or depletion of ARL5A can lead to cellular dysfunction and contribute to disease phenotypes.

Does ARL5A have any known roles in diseases? 01/09/2016

ARL5A has been linked to certain diseases and pathological conditions. Mutations in ARL5A have been associated with Bardet-Biedl syndrome, a rare genetic disorder characterized by various developmental abnormalities and ciliary dysfunction.

Customer Reviews (8)

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Reviews
10/02/2022

    manufacturers or suppliers of proteins can play a crucial role in supporting researchers during trials.

    03/16/2022

      When combined with the support and quality assurance provided by the manufacturer, researchers can confidently utilize ARL5A protein to unravel the intricate mechanisms underlying cellular processes, ultimately advancing scientific knowledge.

      08/20/2021

        The manufacturer not only ensures the provision of superior protein but also aids researchers with their solid technical support, offering the necessary resources to optimize experimental protocols.

        05/03/2021

          the manufacturer can contribute to the research process by providing top-quality ARL5A protein that meets rigorous standards of purity, stability, and reliability.

          10/27/2020

            Manufacturers may provide documentation of the protein's validation, which includes information about its purity, concentration, stability, and potential contaminants.

            04/05/2019

              As a researcher, utilizing ARL5A protein in trials offers numerous advantages that contribute to the successful completion of experiments and the acquisition of valuable data.

              11/09/2017

                ARL5A, a calcium-dependent phospholipid-binding protein, plays a crucial role in various cellular processes, making it a valuable tool for investigation.

                03/20/2017

                  If the need arises for larger quantities of ARL5A protein during the later stages of clinical trials, the manufacturer can facilitate scale-up production processes to meet those requirements.

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