Recombinant Human ARIH1 293 Cell Lysate
Cat.No. : | ARIH1-8726HCL |
- Specification
- Gene Information
- Related Products
Description : | Antigen standard for ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila) (ARIH1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | ARIH1 ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila) [ Homo sapiens ] |
Official Symbol : | ARIH1 |
Synonyms : | ARIH1; ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila); ariadne (Drosophila) homolog, ubiquitin conjugating enzyme E2 binding protein, 1; E3 ubiquitin-protein ligase ARIH1; ARI; ariadne; Drosophila; homolog of; HARI; HHARI; UBCH7BP; ARI-1; MOP-6; H7-AP2; monocyte protein 6; ubcH7-binding protein; protein ariadne-1 homolog; ubcM4-interacting protein; ariadne, Drosophila, homolog of; ubiquitin-conjugating enzyme E2-binding protein 1; FLJ20329; FLJ93118; DKFZp686O13120; |
Gene ID : | 25820 |
mRNA Refseq : | NM_005744 |
Protein Refseq : | NP_005735 |
MIM : | 605624 |
UniProt ID : | Q9Y4X5 |
Chromosome Location : | 15q24 |
Pathway : | Antiviral mechanism by IFN-stimulated genes, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; ISG15 antiviral mechanism, organism-specific biosystem; Immune System, organism-specific biosystem; Interferon Signaling, organism-specific biosystem; |
Function : | ligase activity; metal ion binding; protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; zinc ion binding; |
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
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Q&As (12)
Ask a questionYes, the ARIH1 protein contains several functional domains, including an N-terminal RBR (RING-between-RING) domain, which is responsible for its E3 ubiquitin ligase activity. It also has a central coiled-coil domain and a C-terminal region that contributes to its protein-protein interactions. These domains collectively contribute to the diverse functions of the ARIH1 protein.
Yes, the ARIH1 protein interacts with various proteins and cofactors to carry out its functions. Some known interacting proteins include BRCA1, TRAF6, and UbcH7. These interactions play a role in modulating ARIH1 activity and its involvement in different cellular pathways.
The ARIH1 protein is known to play a role in innate immune signaling by regulating the activation of the NF-κB pathway. It interacts with and promotes the ubiquitination and degradation of proteins involved in NF-κB signaling, thereby controlling the immune response.
Yes, mutations in the ARIH1 gene have been associated with a rare autosomal recessive disorder known as autoinflammation with arthritis and dyskeratosis (AIADK). This condition is characterized by recurrent fever episodes, arthritis, and other systemic symptoms.
The involvement of the ARIH1 protein in neurodegenerative diseases is still an area of ongoing research. Some studies suggest that dysregulation of ARIH1 may contribute to the pathogenesis of neurodegenerative diseases, such as Parkinson's disease. However, more research is needed to fully understand its role in these conditions.
Currently, there are no specific drugs that directly target the ARIH1 protein. However, understanding its role in disease pathways may help identify potential therapeutic strategies indirectly targeting ARIH1-associated conditions.
The ARIH1 protein is actively being studied in several areas of research. Some ongoing areas of investigation include its role in cancer development and progression, its involvement in cellular stress responses, and its impact on protein homeostasis. Further research is needed to fully understand its contributions in these contexts.
Yes, the ARIH1 protein has been found to be involved in mitochondrial quality control. It helps in the removal of damaged or dysfunctional mitochondria through a process called mitophagy, ensuring the maintenance of cellular homeostasis.
Mutations in the ARIH1 gene have been associated with AIADK, a rare autosomal recessive disorder. These mutations lead to the loss of ARIH1 function, resulting in dysregulation of immune responses and inflammatory manifestations seen in AIADK patients.
Yes, studies suggest that the ARIH1 protein is involved in DNA damage repair pathways. It interacts with other proteins involved in DNA repair, such as BRCA1, and contributes to the maintenance of genomic stability.
Yes, mutations in the ARIH1 gene can be inherited in an autosomal recessive manner. This means that an individual must inherit two copies of the mutated gene, one from each parent, in order to develop the associated disorder or condition.
ARIH1 functions as an E3 ubiquitin ligase, which means it catalyzes the transfer of ubiquitin molecules onto target proteins. This process marks the proteins for degradation by the proteasome, a cellular recycling machinery.
Customer Reviews (8)
Write a reviewExtensive research has been conducted on ARIH1, making it relatively accessible and well-understood in terms of its structure and functions.
The manufacturer's excellent technical support, commitment to innovation, and customer-centric approach further reinforce its suitability for my research.
The manufacturer's commitment to providing exceptional technical support ensures that I will receive valuable assistance when facing challenges in my research.
They actively engage with researchers, comprehending their specific experimental needs, and offering tailored services accordingly.
Their knowledgeable team is readily available to address any concerns or difficulties I may encounter during my experiments. With their expertise, I can confidently overcome any obstacles and achieve optimal results.
the ARIH1 protein offers superior quality and reliability, making it a perfect fit for my experimental needs.
With their support, I can confidently explore the intricacies of ARIH1 and make significant contributions to the understanding of its functions in various biological processes.
Its versatility allows for its effective utilization in a broad range of studies, including molecular biology, cellular assays, and protein interactions analysis.
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