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Recombinant Human ARF1 293 Cell Lysate

Cat.No. : ARF1-8761HCL
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  • Gene Information
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Description : Antigen standard for ADP-ribosylation factor 1 (ARF1), transcript variant 4 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ARF1 ADP-ribosylation factor 1 [ Homo sapiens ]
Official Symbol : ARF1
Synonyms : ARF1; ADP-ribosylation factor 1;
Gene ID : 375
mRNA Refseq : NM_001658
Protein Refseq : NP_001649
MIM : 103180
UniProt ID : P84077
Chromosome Location : 1q42.13
Pathway : Arf1 pathway, organism-specific biosystem; Arf6 downstream pathway, organism-specific biosystem; COPI Mediated Transport, organism-specific biosystem; Class I PI3K signaling events, organism-specific biosystem; Clathrin derived vesicle budding, organism-specific biosystem; Disease, organism-specific biosystem; Golgi Associated Vesicle Biogenesis, organism-specific biosystem;
Function : GTP binding; GTPase activity; nucleotide binding; protein binding; receptor signaling protein activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (21)

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Can ARF1 be regulated by post-translational modifications? 03/12/2023

Yes, ARF1 can undergo various post-translational modifications, including phosphorylation, acetylation, and lipidation. These modifications can affect its activity, localization, and interactions with other proteins, providing a means of regulation and modulation in response to cellular cues and signaling events.

How is ARF1 protein implicated in cancer? 02/11/2023

Dysregulation of ARF1 has been observed in some cancers, where it can contribute to abnormal membrane trafficking and facilitate tumor cell proliferation, invasion, and metastasis. Targeting ARF1 and its associated pathways may hold therapeutic potential in cancer treatment.

What cellular processes are regulated by ARF1 protein? 04/06/2022

ARF1 regulates membrane trafficking pathways, including protein sorting, vesicle formation, and transport between organelles such as the Golgi apparatus, plasma membrane, and endosomes.

Can ARF1 play a role in neuronal processes? 10/16/2021

Yes, ARF1 is involved in various neuronal processes, including axonal development, synapse formation, and neurotransmitter release. It regulates the trafficking and recycling of synaptic vesicles, ensuring the proper function of neuronal communication.

Can ARF1 protein be targeted for therapeutic purposes? 10/10/2021

Yes, ARF1 and its regulators are potential targets for therapeutic intervention in diseases involving abnormal membrane trafficking, such as cancer and neurodegenerative disorders. Modulating ARF1 activity could potentially impact these processes.

Are there any known diseases caused by ARF1 mutations? 07/27/2021

Although rare, mutations in ARF1 have been associated with certain genetic disorders. For example, mutations in ARF1 have been linked to arthrogryposis, renal dysfunction, and cholestasis syndrome (ARC) characterized by multisystemic impairments.

Can ARF1 protein be regulated by signaling pathways? 06/13/2021

Yes, ARF1 activity can be modulated by various signaling pathways. For example, certain protein kinases can phosphorylate ARF1, leading to its activation or inactivation, depending on the context.

What are the regulators of ARF1 activation? 10/25/2020

ARF1 activation is regulated by guanine nucleotide exchange factors (GEFs) that facilitate the exchange of GDP for GTP, and GTPase-activating proteins (GAPs) that promote GTP hydrolysis, inactivating ARF1.

How is ARF1 involved in membrane recycling? 10/19/2019

ARF1 is essential for the recycling of membranes and receptors within the cell. It regulates the formation of endosomal compartments and mediates the sorting and retrieval of cargo molecules from endosomes back to the plasma membrane or other intracellular compartments.

Are there any diseases associated with abnormalities in ARF1 protein? 04/22/2019

Yes, mutations or dysregulation of ARF1 have been implicated in various diseases, including certain types of cancer, neurodegenerative disorders, and metabolic diseases.

Are there any drugs or compounds that specifically target ARF1? 04/18/2019

Currently, there are no drugs specifically designed to target ARF1. However, certain compounds, such as brefeldin A, have been shown to inhibit ARF1 function by disrupting its activation and guanine nucleotide exchange factors (GEFs). These compounds have been used as research tools to study ARF1 function and vesicular trafficking in cellular processes.

How does ARF1 protein regulate cargo sorting? 01/24/2019

ARF1 interacts with adaptor proteins, such as AP-1 and AP-3, to facilitate the recognition and targeting of specific cargo molecules. This ensures that the appropriate cargo is packaged into vesicles for transport to their intended destinations.

Can ARF1 be regulated by lipid signaling? 09/22/2018

Yes, ARF1 can be regulated by lipid signaling pathways. Certain lipids, such as phosphoinositides, can directly bind and modulate the activity of ARF1 and its associated factors, influencing vesicle formation and cargo sorting.

How is ARF1 protein involved in endocytosis? 07/19/2018

ARF1 is involved in clathrin-mediated endocytosis, a process in which extracellular molecules are internalized by cells. It helps regulate the formation of clathrin-coated pits, which are responsible for the uptake of specific cargo molecules.

How is ARF1 protein involved in protein trafficking within the Golgi apparatus? 06/04/2018

ARF1 plays a central role in the maintenance of Golgi structure and function. It promotes the recruitment of coat proteins to specific Golgi compartments, enabling the formation of transport vesicles and the sorting of proteins for proper secretion or delivery to other organelles.

Does ARF1 protein have any post-translational modifications? 03/02/2017

Yes, like many other proteins, ARF1 undergoes post-translational modifications. One common modification is lipidation, where it is covalently attached to a lipid moiety, which helps anchor it to specific membrane compartments.

Does ARF1 have any interactions with the cytoskeleton? 09/25/2016

Yes, ARF1 has been shown to interact with components of the cytoskeleton, such as actin and microtubules. These interactions play a role in vesicle trafficking and membrane remodeling processes, allowing for the movement of vesicles along the cytoskeletal network.

Are there any known interacting partners of ARF1 protein? 07/19/2016

ARF1 interacts with various effector proteins, such as coatomer protein complex I (COPI), clathrin, and adaptor proteins like AP-1 and AP-3, which mediate vesicle formation and cargo selection.

How is ARF1 protein involved in vesicle formation? 05/15/2016

ARF1 plays a crucial role in the formation of coated vesicles. It recruits coat proteins, such as COPI and clathrin, to specific membranes, initiating the budding process and ultimately leading to the formation of vesicles.

How is ARF1 involved in lipid metabolism? 03/13/2016

ARF1 plays a role in lipid metabolism by regulating the transport and sorting of lipids within cells. It helps control the intracellular trafficking of lipid droplets, contributes to the formation of lipid rafts, and influences the activity of lipid-modifying enzymes, impacting lipid homeostasis and metabolism.

Can ARF1 protein interact with other small GTPases? 01/02/2016

Yes, ARF1 can interact with other small GTPases, such as Rho and Rab proteins, to coordinate and regulate various cellular processes. These interactions provide a means of crosstalk and integration between different signaling pathways.

Customer Reviews (8)

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Reviews
12/01/2021

    The manufacturer's support has played a vital role in my work, providing expert guidance and troubleshooting assistance whenever needed.

    11/12/2021

      Its reliable bioactivity makes it an ideal choice for a diverse range of experimental applications.

      06/09/2020

        Its reliability, specificity, and consistent performance enable me to obtain accurate data to further my research goals.

        04/17/2019

          The manufacturer offers exceptional technical assistance, guiding researchers through various stages of their experiments.

          09/04/2018

            By providing comprehensive data and resources, the manufacturer enables researchers to design and implement their trials with confidence, ensuring the desired outcomes.

            07/27/2017

              the ARF1 protein offers exceptional advantages in my trials, facilitating my exploration of important cellular mechanisms.

              02/09/2017

                This information is crucial for researchers in understanding the protein's behavior and interaction within their specific experimental context.

                10/14/2016

                  I am grateful for their dedication and commitment to ensuring the success of my experiments.

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