Recombinant Human BRWD3, His&FLAG-tagged

• Cat.No.: BRWD3-39H
• Product Overview: Recombinant Human BRWD3 corresponding to amino acids 1295-1443 that contains the bromodomain sequences of BRWD3 (accession number NP_694984.4) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag.

Specification

• Description: Bromodomain and WD repeat domain containing 3 (BRWD3) is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD) residues which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains a bromodomain and multiple WD repeats. The bromodomain functions as a "reader" of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. Mutations in BRWD3 cause mental retardation X-linked type 93, which is also referred to as mental retardation X-linked with macrocephaly. This gene is also associated with translocations in patients with B-cell chronic lymphocytic leukemia (B-CLL).
• Source: E. coli
• Species: Human
• Tag: His&FLAG
• Form: 25 mM HEPES pH 7.5, 150 mM NaCl and 5% glycerol.
• Molecular Mass: 23.37 kDa
• Applications: Binding assays, inhibitor screening, and selectivity profiling.
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Concentration: 2.9 mg/ml

Gene Information

• Gene Name: BRWD3 bromodomain and WD repeat domain containing 3 [ Homo sapiens ]
• Official Symbol: BRWD3
• Synonyms: BRWD3; bromodomain and WD repeat domain containing 3; bromodomain and WD repeat-containing protein 3; FLJ38568; MRX93; novel WD repeat domain protein; bromo domain-containing protein disrupted in leukemia; BRODL; FLJ33254
• Gene ID: 254065
• mRNA Refseq: NM_153252
• Protein Refseq: NP_694984
• MIM: 300553
• UniProt ID: Q6RI45
• Chromosome Location: Xq21.1