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Native Human lys-Plasminogen

Cat.No.: PLG-55H
Product Overview: Native glu-plasminogen is prepared from fresh frozen human plasma by a modification of the procedure of Castellino, utilizing gel filtration and affinity chromatography.
Description: Plasminogen is a single chain glycoprotein zymogen which is synthesized in the liver and circulates in plasma at a concentration of approximately 2.4 μM. The plasminogen molecule contains 790 amino acids, 24 disulfide bridges, no free sulfhydryls and 5 regions of internal sequence homology, known as kringles, between Lys77 and Arg560. These five triple-looped, three disulfide bridged, kringle regions are homologous to the kringle domains in t-PA, u-PA and prothrombin. Plasminogen contains one high affinity (Kd=9x10-6M) and four low affinity (Kd=5x10-3M) lysine binding sites. The high affinity binding site resides within the first kringle region of plasminogen. The interaction of plasminogen with fibrin and α2-antiplasmin is mediated by these lysine binding sites. Native glu-plasminogen (Mr=88,000) is readily converted to Lys-77-plasminogen (Mr=83,000) by plasmin hydrolysis of the Lys76-Lys77 peptide bond. Elastase catalyzed cleavage of the Val441-Val442 peptide bond of glu-plasminogen yields a functionally active zymogen termed Val-442 plasminogen or mini-plasminogen. The conversion of plasminogen to plasmin occurs by a variety of mechanisms, but all result in hydrolysis of the Arg560-Val561 peptide bond of plasminogen, yielding two chains which remain covalently associated by a disulfide bond.
Species: Human
Form: 50% (vol/vol) glycerol/H2O
Molecular Mass: 83,000
Purity: >95% by SDS-PAGENOT tissue/cell culture grade. Not tested for endotoxin.
Gene Name: PLG plasminogen [ Homo sapiens ]
Official Symbol: PLG
Synonyms: PLG; plasminogen; plasmin; DKFZp779M0222;
Gene ID: 5340
mRNA Refseq: NM_001168338
Protein Refseq: NP_001161810
MIM: 173350
UniProt ID: P00747
Chromosome Location: 6q26
Pathway: Activation of Matrix Metalloproteinases, organism-specific biosystem; Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem; Blood Clotting Cascade, organism-specific biosystem; Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Degradation of the extracellular matrix, organism-specific biosystem;
Function: apolipoprotein binding; cell surface binding; peptidase activity; protein binding; protein domain specific binding; serine-type endopeptidase activity;

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