Active Native Mouse Factor X

• Cat.No.: F10-290M

Specification

• Description: Factor X is a vitamin K-dependent protein zymogen which is synthesized in the liver and circulates in plasma as a two chain molecule linked by a disulfide bond. Prior to secretion into plasma, post-translational modifications produce 11 gamma-carboxyglutamic acid (gla) residues and a single b-hydroxyaspartic acid residue, which are located within the NH2-terminal light chain. The light chain also contains two epidermal growth factor (EGF) homology domains. The COOH-terminal heavy chain of factor X contains most of the carbohydrate moieties, as well as the latent serine protease domain. The activation of factor X is catalyzed by either the intrinsic factor Xase complex (factor IXa, factor VIIIa, cellular surface and calcium ions) or the extrinsic factor Xase complex (factor VIIa, tissue factor, cellular surface and calcium ions). Activation of human factor X by either complex results in cleavage at Arg52-Ile53 of the COOH-terminal heavy chain and subsequent release of a 52 amino acid activation glycopeptide. Factor Xa then serves as the enzyme component of the prothrombinase complex which is responsible for the rapid conversion of prothrombin to thrombin. The gla residues enable factor X/Xa to bind phospholipid (i.e. cell surfaces) in a calcium dependent manner; a requirement for assembly of the prothrombinase complex. The first EGF homology domain contains a Ca2+ binding site which acts as a hinge to fold the EGF and GLA domains towards each other. This region of the molecule is involved in the recognition of cellular binding domains.
• Source: Plasma
• Species: Mouse
• Form: 50% (vol/vol) glycerol/H2O
• Bio-activity: Clotting assay
• Purity: >95% by SDS-PAGE. NOT tissue/cell culture grade. Not tested for endotoxin.
• Characteristic: Plasma concentration:10 μg/ml
• Storage: -20°C

Gene Information

• Gene Name: F10 coagulation factor X [ Mus musculus ]
• Official Symbol: F10
• Synonyms: fX; Cf10; coagulation factor X; stuart factor
• Gene ID: 14058
• mRNA Refseq: NM_001242368
• Protein Refseq: NP_001229297
• Chromosome Location: 8 A1.1; 8 5.73 cM
• Pathway: Blood Clotting Cascade, organism-specific biosystem; Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem
• Function: calcium ion binding; catalytic activity; hydrolase activity