Active Native Mouse Antithrombin III

• Cat.No.: Serpinc1-298M

Specification

• Description: Antithrombin III (ATIII) is a single chain glycoprotein with a molecular weight of 58,000. It is a member of the serpin (serine protease inhibitor) super family and is considered to be the most important inhibitor in the coagulation cascade. ATIII inhibits a wide spectrum of serine proteases including thrombin, factors IXa, Xa and XIa, kallikrein, plasmin, urokinase, C1-esterase, and trypsin. The mechanism of inhibition involves the formation of a stable 1:1 complex between the active site of the protease and the scissile bond (Arg 385-Ser 386) of ATIII. The active site serine of thrombin has been shown to form a covalent intermediate with the P1 amino acid (Arg 385) of ATIII. The rate of inhibition of serine proteases by ATIII is increased to varying degrees by heparin. In the case of being a thrombin inhibitor or factor Xa inhibitor, the interaction with ATIII is enhanced 3 orders of magnitude in the presence of heparin. The interaction between ATIII and heparin involves a unique sequence of sulfated and non-sulfated monosaccharide units on heparin, and critical lysine residues on ATIII. The binding of ATIII to heparinoid structures on vascular endothelium has been demonstrated and shown to enhance the inhibition of factors IXa, Xa, and thrombin.
• Source: Plasma
• Species: Mouse
• Form: 50% (vol/vol) glycerol/H2O
• Bio-activity: Thrombin inhibition
• Purity: >95% by SDS-PAGE. NOT tissue/cell culture grade. Not tested for endotoxin.
• Characteristic: Extinction coefficient:6.2, Plasma concentration:150 μg/ml, Isoelectric point: 4.9-5.3, Percent carbohydrate:0.09
• Storage: -20°C

Gene Information

• Gene Name: Serpinc1 serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 [ Mus musculus ]
• Official Symbol: Serpinc1
• Synonyms: At3; At-3; ATIII; AI114908; antithrombin-III; serpin C1; antithrombin; anti-thrombin 3; serpin peptidase inhibitor, clade C, member 1; serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1
• Gene ID: 11905
• mRNA Refseq: NM_080844
• Protein Refseq: NP_543120
• UniProt ID: P32261
• Chromosome Location: 1 H2.1; 1 69.75 cM
• Pathway: Common Pathway, organism-specific biosystem; Complement and Coagulation Cascades, organism-specific biosystem; Formation of Fibrin Clot (Clotting Cascade), organism-specific biosystem
• Function: heparin binding; protease binding; serine-type endopeptidase inhibitor activity