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Recombinant Aeromonas Aminopeptidase

Cat. No. : Aminopeptidase-86A
Product Overview : The 29 kDa Aeromonas Aminopeptidase is produced by genetic engineering and can be used for physical & structural investigations, sequence and amino-terminal determinations. This exopeptidase recognizes a specific stop sign at –X- Pro and requires a free a-amino group in the L-configuration. It is therefore suitable for the removal of the redundant N-terminal methionine often added to engineered recombinant proteins.
Source : Aeromonas Proteolytica.
Physical Appearance : Sterile filtered liquid formulation. (446U/ml).
Purity : Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Formulation : Buffered solution containing 10 mM Tris-HCL, 100 mM NaCl and 5 µM ZnSO4, pH 8.0.
Unit Defenition : One unit is defined as the amount of enzyme that hydrolyses 1µmole of L-leucine p-nitroanilide at 25°C per 1 minute.
Biological Activity : Recombinant Aeromonas Aminopeptidase was found to have an activity of 120 Units/mg protein.
Stability : Two years when stored at –20°C, 2 weeks at 4°C.
Synonyms : Aminopeptidase, Bacterial leucyl aminopeptidase, EC 3.4.11.10; Aeromonas proteolytica aminopeptidase; EC 3.4.11.1; EC 3.4.11.5; LAP; LAPEP; PEPS; Leucine aminopeptidase; Leucyl aminopeptidase; Peptidase S; Proline aminopeptidase; Prolyl aminopeptidase; leucine aminopeptidase 3; leucyl aminopeptidase; leucine aminopeptidase; leucyl peptidase; peptidase S; cytosol aminopeptidase; cathepsin III; L-leucine aminopeptidase; leucinaminopeptidase; leucinamide aminopeptidase; FTBL proteins; proteinates FTBL; aminopeptidase II; aminopeptidase III; aminopeptidase I.

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