||Recombinant human Calumenin protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography, 37.2 kDa (317aa).
||Calumenin, also known as CALU, is a multiple EF-hand calcium-binding protein located in endoplasmic reticulum(ER) and sarcoplasmic reticulum (SR) of mammalian tissues. This protein binds 7 calcium ions with a low affinity and is involved in such ER functions as protein folding and sorting.
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MKPTEKKDRV HHEPQLSDKV HNDAQSFDYD HDAFLGAEEA KTFDQLTPEE SKERLGKIVS KIDGDKDGFV TVDELKDWIK FAQKRWIYED VERQWKGHDL NEDGLVSWEE YKNATYGYVL DDPDPDDGFN YKQMMVRDER RFKMADKDGD LIATKEEFTA FLHPEEYDYM KDIVVQETME DIDKNADGFI DLEEYIGDMY SHDGNTDEPE WVKTEREQFV EFRDKNRDGK MDKEETKDWI LPSDYDHAEA EARHLVYESD QNKDGKLTKE EIVDKYDLFV GSQATDFGEA LVRHDEF
||> 90% by SDS - PAGE
||Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol.
||1 mg/ml (determined by Bradford assay)
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.