||Recombinant Dna K protein was overexpressed in E.coli and purified to apparent homogeneity by using conventional column chromatography techniques (163 aa, 17.7 kDa).
||DNAK, originally identified for its DNA replication by bacteriophage l in E. coli is the bacterial hsp70 chaperone. The protein coding region of the substrate binding domain of DNAK (amino acids 385-546) was amplified by PCR and cloned into an E. coli expression vector. The substrate binding domain of DNAK was overexpressed in E. coli and the recombinant protein was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid(Met) is attached at N- terminus.
||Liquid. In 25 mM Tris-HCl buffer (pH 7.5) containing 1 mM EDTA, 2 mM b-mercaptoethanol.
||> 95% by SDS – PAGE.
||1 mg/ml (determined by Bradford assay).
||MDVKDVLLLD VTPLSLGIET MGGVMTTLIA KNTTIPTKHS QVFSTAEDNQ SAVTIHVLQG ERKRAADNKS LGQFNLDGIN PAPRGMPQIE VTFDIDADGI LHVSAKDKNS GKEQKITIKA SSGLNEDEIQ KMVRDAEANA EADRKFEELV QTRNQGDHLL HST
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.