Recombinant E.coli GrpE Heat Shock Protein GrpE-324E

Recombinant E.coli GrpE Heat Shock Protein

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Recombinant E.coli GrpE Heat Shock Protein

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Cat. No. : GrpE-324E
Product Overview : Recombinant GrpE protein was overexpressed in E.coli and purified by using the conventional column chromatography techniques (197 aa, 21.8 kDa).
Description : GrpE, co-chaperone of E.coli, participates actively in the response to hyperosomotic and heat shock by preventing the aggregation of stress-denatured proteins in association with DnaK. This protein is the nucleotide exchange factor for DnaK and may function as a thermosensor. Several rounds of ATP-dependent interactions between DnaJ, Dna K and GrpE are required for fully efficient folding.
Source : E.coli.
Formulation : Liquid. In 20 mM Tris buffer (pH 8.0) containing 100 mM NaCl.
Molecular Weight : 21.8 kDa (197aa).
Purity : > 90% by SDS – PAGE.
Concentration : 1 mg/ml (determined by Bradford assay).
Sequence : MSSKEQKTPE GQAPEEIIMD QHEEIEAVEP EASAEQVDPR DEKIANLEAQ LAEAQTRERD GILRVKAEME NLRRRTELDI EKAHKFALEK FINELLPVID SLDRALEVAD KANPDMSAMV EGIELTLKSM LDVVRKFGVE VIAETNVPLD PNVHQAIAMV ESDDVAPGNV LGIMQKGYTL NGRTIRAAMV TVAKAKA
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name : grpE
Synonyms : grpE; grpE heat shock protein; ECK2610; JW2594; heat shock protein; phage lambda replication; host DNA synthesis; heat shock protein; protein repair.
Gene ID : 947097
Protein Refseq : NP_417104.1
UniProt ID : P09372

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