"GrpE" Related Products

Recombinant E.coli GrpE Heat Shock Protein

Cat. No.: GrpE-324E
Product Overview: Recombinant GrpE protein was overexpressed in E.coli and purified by using the conventional column chromatography techniques (197 aa, 21.8 kDa).
Description: GrpE, co-chaperone of E.coli, participates actively in the response to hyperosomotic and heat shock by preventing the aggregation of stress-denatured proteins in association with DnaK. This protein is the nucleotide exchange factor for DnaK and may function as a thermosensor. Several rounds of ATP-dependent interactions between DnaJ, Dna K and GrpE are required for fully efficient folding.
Source: E.coli.
Formulation: Liquid. In 20 mM Tris buffer (pH 8.0) containing 100 mM NaCl.
Molecular Weight: 21.8 kDa (197aa).
Purity: > 90% by SDS – PAGE.
Concentration: 1 mg/ml (determined by Bradford assay).
Sequence: MSSKEQKTPE GQAPEEIIMD QHEEIEAVEP EASAEQVDPR DEKIANLEAQ LAEAQTRERD GILRVKAEME NLRRRTELDI EKAHKFALEK FINELLPVID SLDRALEVAD KANPDMSAMV EGIELTLKSM LDVVRKFGVE VIAETNVPLD PNVHQAIAMV ESDDVAPGNV LGIMQKGYTL NGRTIRAAMV TVAKAKA
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name: grpE
Synonyms: grpE; grpE heat shock protein; ECK2610; JW2594; heat shock protein; phage lambda replication; host DNA synthesis; heat shock protein; protein repair.
Gene ID: 947097
Protein Refseq: NP_417104.1
UniProt ID: P09372

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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