Recombinant E.coli GrpE Heat Shock Protein

• Cat.No.: GrpE-324E
• Product Overview: Recombinant GrpE protein was overexpressed in E.coli and purified by using the conventional column chromatography techniques (197 aa, 21.8 kDa).

Specification

• Cat. No.: GrpE-324E
• Description: GrpE, co-chaperone of E.coli, participates actively in the response to hyperosomotic and heat shock by preventing the aggregation of stress-denatured proteins in association with DnaK. This protein is the nucleotide exchange factor for DnaK and may function as a thermosensor. Several rounds of ATP-dependent interactions between DnaJ, Dna K and GrpE are required for fully efficient folding.
• Source: E.coli.
• Formulation: Liquid. In 20 mM Tris buffer (pH 8.0) containing 100 mM NaCl.
• Molecular Weight: 21.8 kDa (197aa).
• Purity: > 90% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequence: MSSKEQKTPE GQAPEEIIMD QHEEIEAVEP EASAEQVDPR DEKIANLEAQ LAEAQTRERD GILRVKAEME NLRRRTELDI EKAHKFALEK FINELLPVID SLDRALEVAD KANPDMSAMV EGIELTLKSM LDVVRKFGVE VIAETNVPLD PNVHQAIAMV ESDDVAPGNV LGIMQKGYTL NGRTIRAAMV TVAKAKA
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: grpE
• Synonyms: grpE; grpE heat shock protein; ECK2610; JW2594; heat shock protein; phage lambda replication; host DNA synthesis; heat shock protein; protein repair.
• Gene ID: 947097
• Protein Refseq: NP_417104.1
• UniProt ID: P09372