Recombinant Human SNAP-25 Protein, His tagged

• Cat.No.: SNAP25-296H
• Product Overview: Recombinant Human SNAP-25 Protein with His tag was expressed in E. coli.

Specification

• Description: Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene.
• Source: E. coli
• Species: Human
• Tag: N-His
• Molecular Mass: The protein has a calculated MW of 24.6 kDa.
• AA Sequence: MGSSHHHHHHSSGLVPRGSHMAEDA DMRNELEEMQRRADQLADESLESTR RMLQLVEESKDAGIRTLVMLDEQGE QLERIEEGMDQINKDMKEAEKNLTD LGKFCGLCVCPCNKLKSSDAYKKAW GNNQDGVVASQPARVVDEREQMAIS GGFIRRVTNDARENEMDENLEQVSG IIGNLRHMALDMGNEIDTQNRQIDR IMEKADSNKTRIDEANQ
• Purity: > 90% by SDS-PAGE
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: 1.14 mg/mL
• Storage Buffer: 50 mM HEPES buffer (pH 7.4) containing 5 mM DTT, 100 mM NaCl
• Publication:
  Antigen Detection via the Rate of Ion Current Rectification Change of the Antibody-Modified Glass Nanopore Membrane. (2014)

Gene Information

• Gene Name: SNAP25 synaptosome associated protein 25 [ Homo sapiens (human) ]
• Official Symbol: SNAP25
• Synonyms: SNAP25; synaptosome associated protein 25; SUP; RIC4; SEC9; SNAP; CMS18; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2; synaptosomal-associated protein 25; resistance to inhibitors of cholinesterase 4 homolog; synaptosomal-associated protein, 25kDa; synaptosome associated protein 25kDa
• Gene ID: 6616
• mRNA Refseq: NM_130811
• Protein Refseq: NP_570824
• MIM: 600322
• UniProt ID: P36974