| Description : |
GMCSF is a cytokine that controls the production, differentiation, and function of granul centigradeytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been l centigradealized to a cluster of related genes at chromosome region 5q31, which is known to be ass centigradeiated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granul centigradeytes, macrophages, eosinophils and erythr centigradeytes |
| Source : |
Pichia |
| Species : |
Human |
| Predicted N Terminal : |
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser. |
| Form : |
The protein was lyophilized from a concentrated (1mg/ml) solution containing 10mM phosphate buffer pH 7.0, 40 mg mannitol and 10 mg sucrose. |
| Bio-activity : |
The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is< 0.183 ng/ml, corresponding to a specific activity of 5 x 106> |
| Molecular Mass : |
Granul centigradeyte Macrophage Colony Stimulating Factor Human Recombinant produced in Yeast is a single, glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 26-32 kDa. rhGMCSF differs from the natural human GM-CSF by a substitution of leucine at position 23 (R to L), and the carbohydrate moiety may be different from the native protein. GM-CSF is purified by proprietary chromatographic techniques. |
| Purity : |
Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis bySDS-PAGE. |
| Usage : |
FOR RESEARCH USE ONLY |
| Quality Control Test : |
Lyophilized Granul centigradeyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18 centigrade. Upon reconstitution GMCSF should be stored at 4 centigrade between 2-7 days and for future use below -18 centigrade. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). |
| Concentration : |
1mg/ml |
| Reconstitution : |
It is recommended to reconstitute the lyophilized Granul centigradeyte Macrophage Colony Stimulating Factor in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. |
| Warning : |
Avoid repeated freeze-thaw cycles. |
