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Active Recombinant Human IGFBP-4, Carrier Free

Cat.No. : IGFBP4-8H
Product Overview : Mature IGFBP-4 (Kiefer, M. et al., 1991, J. Biol. Chem. 266:9043 - 9049) (aa residues Asp 22 - Glu 258) was fused to the signal peptide of CD33 and the chimera was expressed in a mouse myeloma cell line, NS0.
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Description : Human IGF binding protein 4 (IGFBP-4) was isolated from human plasma based on its ability to bind immobilized IGF-I. Human IGFBP-4 cDNA encodes a 258 amino acid (aa) residue precursor protein with a predicted 21 aa residue signal peptide that is pr centigradeessed to generate the 237 aa residue mature human IGFBP-4. The human IGFBP-4 contains a potential N-linked glycosylation site and shares approximately 90% amino acid sequence identity with both the mouse and rat IGFBP-4. According to the nomenclature of IGFBPs defined at the 4th International Symposium of IGFs (1997, Tokyo), six high-affinity IGF binding proteins (IGFBP-1, -2, -3, -4, -5, -6), and four IGFBP-related proteins (IGFBPr-1, - 2, -3, -4) have now been identified. All IGFBPs have a high cysteine content and share conserved cysteine residues that are clustered in the amino- and carboxy-terminal thirds of the molecule.
Source : Mammalian cells
Species : Human
Form : Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.
Bio-activity : Measured by its ability to inhibit the biological activity of rhIGF-I or rhIGF-II on MCF-7 cells (Karey, K.P. et al., 1988, Cancer Research 48:4083). The ED50 for this effect is typically 0.03 - 0.09 μg/mL in the presence of 14 ng/mL rhIGF-II.
Molecular Mass : Met 17 from the CD33 signal peptide was retained in the recombinant mature human IGFBP-4. The 238 amino acid residue recombinant mature human IGFBP-4 has a calculated molecular mass of approximately 26 kDa. As a result of glycosylation, the recombinant protein migrates as a 32 kDa and 25 kDa protein under reducing and non-reducing conditions, respectively.
Endotoxin : < 1.0 eu per 1 μg of the cytokine as determined by the lal
Purity : >97%, as determined by SDS-PAGE and visualized by silver stain.
Usage : FOR RESEARCH USE ONLY
Quality Control Test : Lyophilized samples are stable for up to six months at -20 centigrade to -70 centigrade. Upon reconstitution, this cytokine, in the presence of a carrier protein, can be stored under sterile conditions at 2 - 8 centigrade for one month or at -20 centigrade to -70 centigrade in a manual defrost freezer for three months without detectable loss of activity.
Reconstitution : It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a st centigradek solution of no less than 25 μg/mL.
Warning : Avoid repeated freeze-thaw cycles.
Gene Name : IGFBP4 insulin-like growth factor binding protein 4 [ Homo sapiens ]
Official Symbol : IGFBP4
Synonyms : IGFBP4; insulin-like growth factor binding protein 4; insulin like growth factor binding protein 4; insulin-like growth factor-binding protein 4; BP 4; HT29 IGFBP; IBP4; IGF binding protein 4; IGFBP 4; IBP-4; IGF-binding protein 4; BP-4; IGFBP-4; HT29-IGFBP;
Gene ID : 3487
mRNA Refseq : NM_001552
Protein Refseq : NP_001543
MIM : 146733
UniProt ID : P22692
Chromosome Location : 17q12-q21.1
Pathway : Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem; Myometrial Relaxation and Contraction Pathways, organism-specific biosystem; Regulation of Insulin-like Growth Factor (IGF) Activity by Insulin-like Growth Factor Binding Proteins (IGFBPs), organism-specific biosystem; Wnt signaling network, organism-specific biosystem;
Function : insulin-like growth factor binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What role does IGFBP4 play in bone growth and development, particularly in regulating osteoblast and osteoclast activity? 09/09/2022

IGFBP4 plays a key role in bone development, regulating bone-forming and bone-resorbing cells.

What is the impact of IGFBP4 on cardiovascular health, particularly in atherosclerosis and heart disease? 04/30/2022

IGFBP4 impacts cardiovascular health, influencing atherosclerosis and heart disease processes.

What are the effects of IGFBP4 dysregulation on aging and age-related diseases? 10/08/2019

Dysregulation of IGFBP4 is linked to aging and the progression of age-related diseases.

How does IGFBP4 contribute to metabolic regulation and insulin sensitivity? 11/25/2018

IGFBP4 is involved in metabolic regulation, affecting insulin sensitivity and glucose metabolism.

How does IGFBP4 modulate the activity of insulin-like growth factors (IGFs) in various tissues? 10/23/2018

IGFBP4 modulates IGF activity, affecting growth and metabolic functions in tissues.

How do alterations in IGFBP4 expression or function affect wound healing and tissue repair? 07/09/2018

Changes in IGFBP4 expression influence wound healing and tissue repair mechanisms.

How does IGFBP4 influence cancer progression, especially in hormone-dependent cancers? 05/26/2018

IGFBP4 influences cancer progression, particularly in cancers sensitive to hormonal changes.

Customer Reviews (3)

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Reviews
02/25/2019

    Accurate protein structural analysis, crucial for our investigations.

    10/23/2018

      Exceptional protein expression optimization support, greatly appreciated.

      06/06/2018

        Reliable mass spectrometry analysis, pivotal for proteomics studies.

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