Recombinant Streptomyces Avidinii Streptavidin, His-tagged Streptavidin-390S

Recombinant Streptomyces Avidinii Streptavidin, His-tagged

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Recombinant Streptomyces Avidinii Streptavidin, His-tagged

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Cat. No. : Streptavidin-390S
Product Overview : Recombinant His tagged streptavidin was expressed in E.coli and purified by using conventional chromatography techniques,17 kDa (167 aa).
Description : Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin; the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
Sequences of amino acids : MVHHHHHHDP SKDSKAQVSA AEAGITGTWY NQLGSTFIVT AGADGALTGT YESAVGNAES RYVLTGRYDS APATDGSGTA LGWTVAWKNN YRNAHSATTW SGQYVGGAEA RINTQWLLTS GTTEANAWKS TLVGHDTFTK VKPSAASIDA AKKAGVNNGN PLDAVQQ
Formulation : Liquid. In 20 mM Tris-HCl buffer (pH 7.5)
Purity : > 95% by SDS - PAGE
Concentration : 1 mg/ml (determined by Bradford assay)
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

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