Recombinant Streptomyces Avidinii Streptavidin, His-tagged

• Cat.No.: Streptavidin-390S
• Product Overview: Recombinant His tagged streptavidin was expressed in E.coli and purified by using conventional chromatography techniques,17 kDa (167 aa).

Specification

• Cat. No.: Streptavidin-390S
• Description: Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin; the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
• Sequences of amino acids: MVHHHHHHDP SKDSKAQVSA AEAGITGTWY NQLGSTFIVT AGADGALTGT YESAVGNAES RYVLTGRYDS APATDGSGTA LGWTVAWKNN YRNAHSATTW SGQYVGGAEA RINTQWLLTS GTTEANAWKS TLVGHDTFTK VKPSAASIDA AKKAGVNNGN PLDAVQQ
• Formulation: Liquid. In 20 mM Tris-HCl buffer (pH 7.5)
• Purity: > 95% by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.