| Cat.No. : |
xis-56B |
| Product Overview : |
Purified from a strain of E. coli that overexpresses the exonuclease gene from bacteriophage Lambda. |
| Description : |
Lambda Exonuclease is a highly processive 5′→3′ double-stranded exonuclease that degrades one strand of the duplex. Lambda exonuclease can initiate at blunt DNA or DNA containing 3′ single-stranded overhangs. Lambda Exonuclease has greatly reduced activity on non-phosphorylated DNA, single-stranded DNA and DNA having protruding 5′ single-stranded termini. Lambda exonuclease will not initiate at a nick or gap. |
| Source : |
E. coli |
| Species : |
Bacteriophage Lambda |
| Bio-activity : |
80,000 U/mg |
| Molecular Mass : |
25,909 Daltons |
| Purity : |
>99% by SDS-PAGE |
| Unit Definition : |
One unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble deoxyribonucleotide from double-stranded substrate in 30 minutes at 37°C. |
| Storage : |
-25 to -15°C |
| Concentration : |
5,000 U/mL |
| Storage Buffer : |
Supplied in: 25mM Tris-HCl, 50mM NaCl, 1mM DTT, 0.1mM EDTA, 50% glycerol pH 7.5 @ 25?C. Supplied with: 10X Lambda Exo Reaction Buffer: 670 mM Glycine, 25mM MgCl2, 10mM DTT, pH 9.4 @25°C |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.