Recombinant Bacteriophage Lambda Exonuclease xis-56B

Recombinant Bacteriophage Lambda Exonuclease

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Recombinant Bacteriophage Lambda Exonuclease

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Cat.No. : xis-56B
Product Overview : Purified from a strain of E. coli that overexpresses the exonuclease gene from bacteriophage Lambda.
Description : Lambda Exonuclease is a highly processive 5′→3′ double-stranded exonuclease that degrades one strand of the duplex. Lambda exonuclease can initiate at blunt DNA or DNA containing 3′ single-stranded overhangs. Lambda Exonuclease has greatly reduced activity on non-phosphorylated DNA, single-stranded DNA and DNA having protruding 5′ single-stranded termini. Lambda exonuclease will not initiate at a nick or gap.
Source : E. coli
Species : Bacteriophage Lambda
Bio-activity : 80,000 U/mg
Molecular Mass : 25,909 Daltons
Purity : >99% by SDS-PAGE
Unit Definition : One unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble deoxyribonucleotide from double-stranded substrate in 30 minutes at 37°C.
Storage : -25 to -15°C
Concentration : 5,000 U/mL
Storage Buffer : Supplied in: 25mM Tris-HCl, 50mM NaCl, 1mM DTT, 0.1mM EDTA, 50% glycerol pH 7.5 @ 25?C. Supplied with: 10X Lambda Exo Reaction Buffer: 670 mM Glycine, 25mM MgCl2, 10mM DTT, pH 9.4 @25°C
Gene Name : xis exonuclease [ Enterobacteria phage lambda ]
Official Symbol : xis
Synonyms : xis; exonuclease; NP_040616.1
Gene ID : 2703469
Protein Refseq : NP_040616
UniProt ID : 0902231Q

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