Recombinant Bacteriophage Lambda Exonuclease
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| Cat.No. : | xis-56B |
| Product Overview : | Purified from a strain of E. coli that overexpresses the exonuclease gene from bacteriophage Lambda. |
| Description : | Lambda Exonuclease is a highly processive 5′→3′ double-stranded exonuclease that degrades one strand of the duplex. Lambda exonuclease can initiate at blunt DNA or DNA containing 3′ single-stranded overhangs. Lambda Exonuclease has greatly reduced activity on non-phosphorylated DNA, single-stranded DNA and DNA having protruding 5′ single-stranded termini. Lambda exonuclease will not initiate at a nick or gap. |
| Source : | E. coli |
| Species : | Bacteriophage Lambda |
| Bio-activity : | 80,000 U/mg |
| Molecular Mass : | 25,909 Daltons |
| Purity : | >99% by SDS-PAGE |
| Unit Definition : | One unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble deoxyribonucleotide from double-stranded substrate in 30 minutes at 37°C. |
| Storage : | -25 to -15°C |
| Concentration : | 5,000 U/mL |
| Storage Buffer : | Supplied in: 25mM Tris-HCl, 50mM NaCl, 1mM DTT, 0.1mM EDTA, 50% glycerol pH 7.5 @ 25?C. Supplied with: 10X Lambda Exo Reaction Buffer: 670 mM Glycine, 25mM MgCl2, 10mM DTT, pH 9.4 @25°C |
| Gene Name : | xis exonuclease [ Enterobacteria phage lambda ] |
| Official Symbol : | xis |
| Synonyms : | xis; exonuclease; NP_040616.1 |
| Gene ID : | 2703469 |
| Protein Refseq : | NP_040616 |
| UniProt ID : | 0902231Q |
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