Active Recombinant Marine bacteria marine bacteria

• Cat.No.: UDG-59B
• Product Overview: A recombinant E. coli strain carrying the recombinant thermolabile Uracil DNA Glycosylase gene isolated from a marine bacteria.

Specification

• Description: Thermolabile Uracil-DNA Glycosylase removes uracil from DNA by hydrolyzing the N-glycosylic bond between the deoxyribose and the base leaving an AP (apurinic or apyrimidinic) site. This enzyme ( 1-10 units) is completely inactivated by a 10 minute incubation at temperatures greater than 50°C in the 1x reaction buffer as measured in the unit characterization assay.
• Source: E. coli
• Species: Marine bacteria
• Bio-activity: 30,000 U/mg
• Molecular Mass: 26,218 Daltons
• Purity: >98% by SDS-PAGE
• Unit Definition: 1 unit is defined as the amount of Thermolabile UDG required to release 1nmol of Uracil from dU-containing DNA in one hour at 37°C.
• Storage: -25 to -15°C
• Concentration: 1,000 U/ml
• Storage Buffer: Supplied in: 50mM Tris-HCl, 50mM NaCl, 1mM DTT, 0.1mM EDTA, 50% glycerol pH 7.5 @ 25?C. Supplied with: 10X Reaction Buffer: 700mM Tris-HCl, 100mM NaCl, 10mM EDTA, 1 mg/mL BSA pH 8.0 @ 25?C