Recombinant Marine bacteria marine bacteria UDG-59B

Recombinant Marine bacteria marine bacteria

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Recombinant Marine bacteria marine bacteria

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Cat.No. : UDG-59B
Product Overview : A recombinant E. coli strain carrying the recombinant thermolabile Uracil DNA Glycosylase gene isolated from a marine bacteria.
Description : Thermolabile Uracil-DNA Glycosylase removes uracil from DNA by hydrolyzing the N-glycosylic bond between the deoxyribose and the base leaving an AP (apurinic or apyrimidinic) site. This enzyme ( 1-10 units) is completely inactivated by a 10 minute incubation at temperatures greater than 50°C in the 1x reaction buffer as measured in the unit characterization assay.
Source : E. coli
Species : Marine bacteria
Bio-activity : 30,000 U/mg
Molecular Mass : 26,218 Daltons
Purity : >98% by SDS-PAGE
Unit Definition : 1 unit is defined as the amount of Thermolabile UDG required to release 1nmol of Uracil from dU-containing DNA in one hour at 37°C.
Storage : -25 to -15°C
Concentration : 1,000 U/ml
Storage Buffer : Supplied in: 50mM Tris-HCl, 50mM NaCl, 1mM DTT, 0.1mM EDTA, 50% glycerol pH 7.5 @ 25?C. Supplied with: 10X Reaction Buffer: 700mM Tris-HCl, 100mM NaCl, 10mM EDTA, 1 mg/mL BSA pH 8.0 @ 25?C

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