Recombinant S. acidocaldarius Thermostable Pyrophosphatase PPA-60S

Recombinant S. acidocaldarius Thermostable Pyrophosphatase


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Recombinant S. acidocaldarius Thermostable Pyrophosphatase

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Cat.No. : PPA-60S
Product Overview : A recombinant E. coli strain carrying the Thermostable Pyrophosphatase gene from S. acidocaldarius.
Description : Thermostable pyrophosphatase is a recombinant enzyme from Sulfolobus acidocaldarius which catalyzes the Mg-dependent reaction of P2O7-4 + H2O → 2HPO4-2. It is has a low Km (5.4 uM) for pyrophosphate, is active between pH 7 and 9, has an optimal temperature for activity at 75°C and is functional under PCR conditions.
Source : E. coli
Species : S. acidocaldarius
Bio-activity : 3,500 U/mg
Molecular Mass : 19,381 Daltons
Purity : >95% by SDS-PAGE
Unit Definition : One unit is the amount of enzyme that will liberate 1 μmol of phosphate per minute from inorganic pyrophosphate at 75°C and pH 8.5
Storage : -25 to -15°C
Concentration : 2,000 U/ml
Storage Buffer : Supplied in: 10 mM Tris-HCl pH 7.5, 50 mM NaCl, 50% Glycerol, 1 mM DTT, 0.1 mM EDTA

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