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Active Recombinant S. acidocaldarius Thermostable Pyrophosphatase

Cat.No. : PPA-60S
Product Overview : A recombinant E. coli strain carrying the Thermostable Pyrophosphatase gene from S. acidocaldarius.
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Description : Thermostable pyrophosphatase is a recombinant enzyme from Sulfolobus acidocaldarius which catalyzes the Mg-dependent reaction of P2O7-4 + H2O → 2HPO4-2. It is has a low Km (5.4 uM) for pyrophosphate, is active between pH 7 and 9, has an optimal temperature for activity at 75°C and is functional under PCR conditions.
Source : E. coli
Species : S. acidocaldarius
Bio-activity : 3,500 U/mg
Molecular Mass : 19,381 Daltons
Purity : >95% by SDS-PAGE
Unit Definition : One unit is the amount of enzyme that will liberate 1 μmol of phosphate per minute from inorganic pyrophosphate at 75°C and pH 8.5
Storage : -25 to -15°C
Concentration : 2,000 U/ml
Storage Buffer : Supplied in: 10 mM Tris-HCl pH 7.5, 50 mM NaCl, 50% Glycerol, 1 mM DTT, 0.1 mM EDTA

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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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