Recombinant E. coli Poly(A) Polymerase

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Recombinant E. coli Poly(A) Polymerase

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Cat.No. : PF1-62E
Product Overview : The gene encoding?E. coli?Poly(A) Polymerase expressed from a plasmid in?E. coli.
Description : Poly(A) Polymerase catalyzes the addition of AMP from ATP to the 3′ hydroxyl of RNA. The reaction requires Mg2+ and is template independent.
Source : E. coli
Species : E. coli
Bio-activity : >20,000 U/mg
Molecular Mass : 53,870 Daltons
Purity : >95% by SDS-PAGE
Unit Definition : 1 unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-insoluble material in 10 minutes at 37°C.
Storage : -25 to -15°C
Concentration : 5,000 U/mL
Storage Buffer : Supplied in: 25mM Tris-HCl, 500mM NaCl, 0.1mM DTT, 0.1mM EDTA, 1mM MgCl2, 50% glycerol pH 8.0 @ 25?C. Supplied with: 10X Poly(A) Polymerase Reaction Buffer: 500mM Tris-HCl, 2.5M NaCl, 100mM MgCl2 pH 7.9 @ 25?C.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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