Active Recombinant E. coli Poly(A) Polymerase

• Cat.No.: PF1-62E
• Product Overview: The gene encoding?E. coli?Poly(A) Polymerase expressed from a plasmid in?E. coli.

Specification

• Description: Poly(A) Polymerase catalyzes the addition of AMP from ATP to the 3′ hydroxyl of RNA. The reaction requires Mg2+ and is template independent.
• Source: E. coli
• Species: E. coli
• Bio-activity: >20,000 U/mg
• Molecular Mass: 53,870 Daltons
• Purity: >95% by SDS-PAGE
• Unit Definition: 1 unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-insoluble material in 10 minutes at 37°C.
• Storage: -25 to -15°C
• Concentration: 5,000 U/mL
• Storage Buffer: Supplied in: 25mM Tris-HCl, 500mM NaCl, 0.1mM DTT, 0.1mM EDTA, 1mM MgCl2, 50% glycerol pH 8.0 @ 25?C. Supplied with: 10X Poly(A) Polymerase Reaction Buffer: 500mM Tris-HCl, 2.5M NaCl, 100mM MgCl2 pH 7.9 @ 25?C.