"Potein A" Related Products

MabAffinity rProtein A Agarose High Flow

Cat.no: Potein A-001A
Product Overview: Mab Affinity Protein A Agarose High Flow is a high capacity, chemically stableaffinity medium designed specially for the purification of monoclonal and polyclonal antibodies at laboratory and process scales.
Form: Slurry in 20% Ethanol
Size: 5 mL, 25 mL, 100 mL
Matrix: Highly cross-linked Agarose
Molecular Characterization: Recombinant protein A has a molecular weight of Mr 44 600
Average particle size: 90 μm
Ligand: Protein A
Coupling chemistry: Epoxy
Dynamic binding capacity: >30mg IgG /ml media
Recommended flow rate: 100-300cm/h
Viscosity: Unused media can be stored in the container at a temperature of +4 to +8°C. Ensure that the screw top is fully tightened.Packed columns should be equilibrated in binding buffer containing 20% ethanol to prevent microbial growth.After storage, equilibrate with at least 5 bed volumes of starting buffer before use.
Recommended column height: 5‐20cm
Chemical stability: Stable in all aqueous buffers commonly used in protein A chromatography
pH working range: 3-9
pH CIP range: 2-11
CIP stability: 8M Urea, 6M Gua‐HCl or 1mM NaOH+1MNaCl pH 11, 0.1M acetic acid
Temperature Stability: 2‐40 °C
Elution buffer: 100 mM glycin, 10 mM NaCl, pH 3.0
Shelf life: 5 years
Warning: 1. Determined at 10% breakthrough at 4min residence time2. Delivered at room temperature, and recommended long‐term storage at 2‐8 °C

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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