"Potein A" Related Products

      MabAffinity rProtein A Agarose High Flow

      Cat.no : Potein A-001A
      Product Overview : Mab Affinity Protein A Agarose High Flow is a high capacity, chemically stableaffinity medium designed specially for the purification of monoclonal and polyclonal antibodies at laboratory and process scales.
      Form : Slurry in 20% Ethanol
      Size : 5 mL, 25 mL, 100 mL
      Matrix : Highly cross-linked Agarose
      Molecular Characterization : Recombinant protein A has a molecular weight of Mr 44 600
      Average particle size : 90 μm
      Ligand : Protein A
      Coupling chemistry : Epoxy
      Dynamic binding capacity : >30mg IgG /ml media
      Recommended flow rate : 100-300cm/h
      Viscosity : Unused media can be stored in the container at a temperature of +4 to +8°C. Ensure that the screw top is fully tightened.Packed columns should be equilibrated in binding buffer containing 20% ethanol to prevent microbial growth.After storage, equilibrate with at least 5 bed volumes of starting buffer before use.
      Recommended column height : 5‐20cm
      Chemical stability : Stable in all aqueous buffers commonly used in protein A chromatography
      pH working range : 3-9
      pH CIP range : 2-11
      CIP stability : 8M Urea, 6M Gua‐HCl or 1mM NaOH+1MNaCl pH 11, 0.1M acetic acid
      Temperature Stability : 2‐40 °C
      Elution buffer : 100 mM glycin, 10 mM NaCl, pH 3.0
      Shelf life : 5 years
      Warning : 1. Determined at 10% breakthrough at 4min residence time2. Delivered at room temperature, and recommended long‐term storage at 2‐8 °C

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