"Potein L" Related Products

MabAffinity rProtein L Agarose High Flow

Cat.no: Potein L-001A
Product Overview: MabAffinity Protein L Agarose High Flow Resin, with rProtein L coupled to high flow Agarose matrix, can capture a wide range of antibody and antibody fragments effectively due to its strong affinity to the variable region of antibody kappa light chains.
Form: Slurry in 20% Ethanol
Size: 5 mL, 25 mL, 100 mL
Matrix: Highly cross-linked Agarose
Molecular Characterization: Recombinant Protein L has a mass of 36kDa
Average particle size: 90 μm
Ligand: Protein L
Coupling chemistry: Epoxy
Dynamic binding capacity: >5-10 mg human IgG /ml media
Recommended flow rate: 100-300cm/h
Viscosity: Unused media can be stored in the container at a temperature of +4 to +8°C. Ensure that the screw top is fully tightened and keep container tightly closed in a dry and well-entilated place.Packed columns should be equilibrated in binding buffer containing 20% ethanol to prevent microbial growth.After storage, equilibrate with at least 5 bed volumes of starting buffer before use.
Recommended column height: 5‐20cm
pH working range: 2-10
pH CIP range: 2-11
CIP stability: 8M Urea, 10mM NaOH + 1M NaCl, 0.1Macetic acid
Temperature Stability: 2‐40 °C
Elution buffer: 100 mM glycin, 10 mM NaCl, pH 3.0
Shelf life: 5 years
Warning: 1. Determined at 10% breakthrough at 4min residence time2. Delivered at room temperature, and recommended long‐term storage at 2‐8 °C

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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