||Superose 12 is for high resolution purification of proteins from 1 × 103 to 3 x 105 molecular weight, while Superose 12 Prep Grade is useful for preparative purification of these higher molecular weight proteins.
||Highly cross-linked agarose
|Average particle size:
||30 μm-40 μm
||Store your column in water or buff er containing 20 % ethanol. Avoid prolonged exposures to extremes of pH.
||Resistant to all solutions commonly used in gel filtration, including 8 M urea, 6 M guanidine HCl and 30 % acetronitrile. A packed column should be used in the temperature range 4–40 oC. The loose medium can he repeatedly autoclaved at pH 7, 120 oC. Stable for long periods in the pH range 3–12 and for short periods, such as cleaning, in the pH range 1–14. High concentrations of formic acid should not be used.
||Can stand back-pressures up to 0.7 MPa (7 bar, 105 psi)
|pH working range:
|pH CIP range: