Lambda Fab Select Kit
| Cat.No. : | Lambda-001A |
| Product Overview : | Lambda Fab Select is affinity chromatography media for purifying lambda Fab fragments, which enable efficient capture with high purity and yield. |
- Specification
- Gene Information
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| Cat.no : | Lambda-001A |
| Form : | suspension in 20% ethanol |
| Matrix : | Highly cross-linked agarose |
| Average particle size : | 75 μm |
| Ligand : | Recombinant protein (Mr 13 000), produced in S. cerevisiae, with affinity for the constant domain of the immunoglobulin Fab lambda light chain |
| Ligand concentration : | Approx. 7 mg/ml of medium |
| Dynamic binding capacity : | Approximately 20 mg Fab/mL of medium |
| Recommended flow rate : | At least 600 cm/h in a 1 m diameter column, with 20 cm bed height at 20°C using buffers with the same viscosity as water at< 3> |
| Chemical stability : | Stable to all commonly used aqueous buffers: 6 M urea, 6 M guanidine hydrochloride, 70 % ethanol, 30 % isopropanol |
| pH working range : | 3-10 |
| pH CIP range : | 2-12 |
| Temperature Stability : | 4–30 °C |
| Elution buffer : | 0.1 M acetate buffer, pH 3.5 |
| Warning : | Prolonged exposure (i.e., several days) to pH< 2 should be avoided due to slow decomposition of the agarose matrix at low ph. for kappaselect avoid ph>12. |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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