| Cat.no: | Lambda-001A |
| Product Overview: | Lambda Fab Select is affinity chromatography media for purifying lambda Fab fragments, which enable efficient capture with high purity and yield. |
| Form: | suspension in 20% ethanol |
| Matrix: | Highly cross-linked agarose |
| Average particle size: | 75 μm |
| Ligand: | Recombinant protein (Mr 13 000), produced in S. cerevisiae, with affinity for the constant domain of the immunoglobulin Fab lambda light chain |
| Ligand concentration: | Approx. 7 mg/ml of medium |
| Dynamic binding capacity: | Approximately 20 mg Fab/mL of medium |
| Recommended flow rate: | At least 600 cm/h in a 1 m diameter column, with 20 cm bed height at 20°C using buffers with the same viscosity as water at< 3> |
| Chemical stability: | Stable to all commonly used aqueous buffers: 6 M urea, 6 M guanidine hydrochloride, 70 % ethanol, 30 % isopropanol |
| pH working range: | 3-10 |
| pH CIP range: | 2-12 |
| Temperature Stability: | 4–30 °C |
| Elution buffer: | 0.1 M acetate buffer, pH 3.5 |
| Warning: | Prolonged exposure (i.e., several days) to pH< 2 should be avoided due to slow decomposition of the agarose matrix at low ph. for kappaselect avoid ph>12. |
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