"Lambda" Related Products

Lambda Fab Select Kit

Cat.no: Lambda-001A
Product Overview: Lambda Fab Select is affinity chromatography media for purifying lambda Fab fragments, which enable efficient capture with high purity and yield.
Form: suspension in 20% ethanol
Matrix: Highly cross-linked agarose
Average particle size: 75 μm
Ligand: Recombinant protein (Mr 13 000), produced in S. cerevisiae, with affinity for the constant domain of the immunoglobulin Fab lambda light chain
Ligand concentration: Approx. 7 mg/ml of medium
Dynamic binding capacity: Approximately 20 mg Fab/mL of medium
Recommended flow rate: At least 600 cm/h in a 1 m diameter column, with 20 cm bed height at 20°C using buffers with the same viscosity as water at< 3>
Chemical stability: Stable to all commonly used aqueous buffers: 6 M urea, 6 M guanidine hydrochloride, 70 % ethanol, 30 % isopropanol
pH working range: 3-10
pH CIP range: 2-12
Temperature Stability: 4–30 °C
Elution buffer: 0.1 M acetate buffer, pH 3.5
Warning: Prolonged exposure (i.e., several days) to pH< 2 should be avoided due to slow decomposition of the agarose matrix at low ph. for kappaselect avoid ph>12.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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