Cat.no: | Lambda-001A |
Product Overview: | Lambda Fab Select is affinity chromatography media for purifying lambda Fab fragments, which enable efficient capture with high purity and yield. |
Form: | suspension in 20% ethanol |
Matrix: | Highly cross-linked agarose |
Average particle size: | 75 μm |
Ligand: | Recombinant protein (Mr 13 000), produced in S. cerevisiae, with affinity for the constant domain of the immunoglobulin Fab lambda light chain |
Ligand concentration: | Approx. 7 mg/ml of medium |
Dynamic binding capacity: | Approximately 20 mg Fab/mL of medium |
Recommended flow rate: | At least 600 cm/h in a 1 m diameter column, with 20 cm bed height at 20°C using buffers with the same viscosity as water at< 3> |
Chemical stability: | Stable to all commonly used aqueous buffers: 6 M urea, 6 M guanidine hydrochloride, 70 % ethanol, 30 % isopropanol |
pH working range: | 3-10 |
pH CIP range: | 2-12 |
Temperature Stability: | 4–30 °C |
Elution buffer: | 0.1 M acetate buffer, pH 3.5 |
Warning: | Prolonged exposure (i.e., several days) to pH< 2 should be avoided due to slow decomposition of the agarose matrix at low ph. for kappaselect avoid ph>12. |
Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.