"Protein A" Related Products


TargetLock Protein A Beads

Cat.no: Protein A-33A
Product Overview: The origin of protein A is from the bacteria Staphylococcus aureus. Protein A is capable of binding most classes and subclasses of immunoglobulins from Goat, Rabbit, Mouse and Human. Our Protein A beads are produced using genetically engineered form of Protein A. Nonessential regions have been removed while leaving the immunoglobulins binding sites intact. The recombinant Protein A has been immobilized to Sepharose beads using proprietary TargetLock chemistry, in order to create a winner product for the affinity purification and immunoprecipitation of various mammalian immunoglobulins.
Matrix: Highly cross-linked spherical agarose, 4%
Average particle size: 40‐165 μm
Dynamic binding capacity: ~4-8 mg rabbit IgG per ml of resin
Recommended flow rate: 1-3 ml/min/ cm2
pH working range: 2-11
Elution buffer: 0.1M glycine buffer adjusted pH to 2.8 by acetic acid

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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