|Product Overview:||Recombinant Protein-G used in our Protein G beads is a genetically engineered form of Protein-G. Non-essential regions have been removed while leaving the IgG binding sites intact. The recombinant Protein G has been immobilized on Sepharose beads, using our Biotech's TargetLock chemistry, in order to create a versatile product for both the purification of various mammalian immunoglobulins and for isolating immune complexes by immunoprecipitation (IP).|
|Matrix:||Highly cross-linked spherical agarose, 4%|
|Average particle size:||40‐165 μm|
|Dynamic binding capacity:||~4-8 mg rabbit IgG per ml of beads|
|Recommended flow rate:||1-3 ml/min/ cm2|
|pH working range:||2-11|
|Elution buffer:||0.1M glycine buffer Adjust pH to 2.8 by 1N HCl|
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