"Protein G" Related Products

TargetLock Protein G Beads

Cat.no: Protein G-34A
Product Overview: Recombinant Protein-G used in our Protein G beads is a genetically engineered form of Protein-G. Non-essential regions have been removed while leaving the IgG binding sites intact. The recombinant Protein G has been immobilized on Sepharose beads, using our Biotech's TargetLock chemistry, in order to create a versatile product for both the purification of various mammalian immunoglobulins and for isolating immune complexes by immunoprecipitation (IP).
Matrix: Highly cross-linked spherical agarose, 4%
Average particle size: 40‐165 μm
Dynamic binding capacity: ~4-8 mg rabbit IgG per ml of beads
Recommended flow rate: 1-3 ml/min/ cm2
pH working range: 2-11
Elution buffer: 0.1M glycine buffer Adjust pH to 2.8 by 1N HCl

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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