||Recombinant Human CTH was expressed in E. coli.
||This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in three transcript variants encoding different isoforms.
||70 mM Tris, pH 8.1, containing 150 mM sodium chloride, 20% glycerol, and 10 μM pyridoxal-5"-phosphate.
||≥85% estimated by SDS-PAGE
||One unit is fefined as the amount of enzyme required to produce 1umol of TNB per minute at 30℃ in 50 mM Tris, pH 8.6, containing 20uM pyridoxal-5"-phosphate, and 3mM L-cystathionine substrate.
||Store at -80oC