Active Recombinant Human ARSB, His-tagged

• Cat.No.: ARSB-1062H
• Product Overview: Recombinant Human ARSB(Ser37-Met533), fused with a C-terminal 10-His tag, was expressed in CHO Cells.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: 37-533 a.a.
• Description: Arylsulfatase B encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targetted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
• Predicted N Terminal: Ser37
• Form: Supplied as a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS). The specific activity is >3,000 pmol/min/µg.
• Molecular Mass: Predicted Molecular Mass: 57 kDaSDS-PAGE: 80 kDa, reducing conditions
• Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles.6 months from date of receipt, -20 to -70 ºC as supplied.3 months, -20 to -70 ºC under sterile conditions after opening.

Gene Information

• Gene Name: ARSB arylsulfatase B [ Homo sapiens (human) ]
• Official Symbol: ARSB
• Synonyms: ARSB; arylsulfatase B; ASB; G4S; MPS6; N-acetylgalactosamine-4-sulfatase; NP_000037.2; EC 3.1.6.12; NP_942002.1
• Gene ID: 411
• mRNA Refseq: NM_000046
• Protein Refseq: NP_000037
• MIM: 611542
• UniProt ID: P15848
• Chromosome Location: 5q14.1
• Pathway: CS/DS degradation; Chondroitin sulfate degradation; Chondroitin sulfate/dermatan sulfate metabolism
• Function: N-acetylgalactosamine-4-sulfatase activity; arylsulfatase activity; metal ion binding