Activated Thiol Agarose Fast Flow

• Cat.No.: Thiol-101A
• Product Overview: Activated Thiol Agarose Fast Flow medium is a medium used for reversible immobilization of molecules containing thiol groups under mild conditions. Optimized for immobilization of large molecules. Activated Thiol Agarose Fast Flow reacts with solutes containing thiol groups under mild conditions to form mixed disulphides. This reaction forms the basis of covalent chromatography and a procedure for immobilizing thiol containing biomolecules1. Reversible coupling of proteins and large biomolecules with thiol groups via a glutathione spacer arm2. Well suited for covalent chromatography of large molecules such as enzymes and nucleic acids3. Gel also reacts with heavy metal ions and with alkyl and aryl halides. Addition reactions occur with compounds containing C=O, C=C, and N=N bonds4. Separate thiol-containing proteins from non-thiol-containing proteins

Specification

• Cat.no: Thiol-101A
• Size: 20 ml; 50 ml
• Matrix: cross-linked Agarose
• Functional group: -SH
• Average particle size: 90 µm
• Ligand concentration: 15-30 μmol /ml
• Recommended flow rate: 300 cm/h
• Chemical stability: Stable to all commonly used aqueous buffers and additives like detergents.
• Physical stability: Negligible volume variation due to changes in pH or ionic strength.
• pH working range: 3-10
• Shelf life: Stable for 2 years when stored at -20 °C