Recombinant Pasteurella multocida toxin toxA-143P

Recombinant Pasteurella multocida toxin

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Recombinant Pasteurella multocida toxin

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Cat.No. : toxA-143P
Product Overview : This product is derived from a recombinant ToxA with His6-tag at N-termina expressed in E. coli. Its molecular weight is 145 kDa.
Description : Pasteurella multocida toxin (PMT) is produced by a gram-negative bacillus, Pasteurella multocida. PMT activates the Gq and G12/13 dependent signaling pathways. Gq and G12/13 are alpha subunits of the GTP trimer bound protein of animal cells. It does not activate the highly related G11-dependent pathways. This toxin binds to a ganglioside-type cell surface receptor, acts intracellularly after having been internalized through an endocytic pathway, and has pleiotropic effects on cell physiology. Therefore it does not function on receptor-deficient cells or cells defective in the endocytosis pathway. PMT is encoded by the toxA gene of P. multocida.
Source : E. coli
Species : Pasteurella multocida
Tag : His
Form : 50% glycerol, 5mM Tris-HCl(pH7.5), 0.1M NaCl
Purity : More than 90% purity by SDS-PAGE (CBB staining)
Applications : 1) For the studies on the GTP trimer bound protein- dependent signaling pathway 2) As a PMT antigen for immunological assays such as ELISA, western blotting and dot blotting.
Storage : Shipped at 4℃ or -20℃ and stored at -20℃ or below
Concentration : 370 μg/ml

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