Active Recombinant Human MEP1A, His-tagged

• Cat.No.: MEP1A-156H
• Product Overview: Recombinant Human MEP1A (Accession # AAA21338) Val22-Gln601 & Leu27-Gln601, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Val22 and Leu27
• Form: Supplied as a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH .The specific activity is >500 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human MEP1A, His-tagged has a calculated MW of 67 kDa. In SDS-PAGE migrates as 86 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: MEP1A meprin A, alpha (PABA peptide hydrolase) [ Homo sapiens ]
• Official Symbol: MEP1A
• Synonyms: MEP1A; meprin A, alpha (PABA peptide hydrolase); meprin A subunit alpha; PPHA; PPH alpha; endopeptidase-2; PABA peptide hydrolase; bA268F1.1 (meprin A alpha (PABA peptide hydrolase)); N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha
• Gene ID: 4224
• mRNA Refseq: NM_005588
• Protein Refseq: NP_005579
• MIM: 600388
• UniProt ID: Q16819
• Chromosome Location: 6p12-p11