Active Recombinant F. meningosepticum PNGF, Met&His-tagged

• Cat.No.: PNGF-200F
• Product Overview: Recombinant F. meningosepticum PNGF (Accession # AAA85323) Ala41-Asn354, fused with N-terminal Met and 6-His tag, was produced in E. coli-derived.

Specification

• Species: F.meningosepticum
• Source: E.coli
• Tag: His
• Protein Length: 41-354 a.a.
• Predicted N Terminal: Met
• Form: Supplied as a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to deglycosylate ribonuclease B under denatured conditions.>50% ribonuclease B (10 µg) is deglycosylated by 10 ng of rFmPNGase F within 30 minutes, as measured under the described conditions.
• Molecular Mass: Recombinant F. meningosepticum PNGF, Met&His-tagged has a calculated MW of 36 kDa. In SDS-PAGE migrates as 33-35 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).