Recombinant F. meningosepticum PNGF, Met&His-tagged


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Recombinant F. meningosepticum PNGF, Met&His-tagged

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Cat.No. : PNGF-200F
Product Overview : Recombinant F. meningosepticum PNGF (Accession # AAA85323) Ala41-Asn354, fused with N-terminal Met and 6-His tag, was produced in E. coli-derived.
Source : E. coli
Species : F. meningosepticum
Tag : &His
Predicted N Terminal : Met
Form : Supplied as a 0.2 µm filtered solution in Tris and NaCl.
Bio-activity : Measured by its ability to deglycosylate ribonuclease B under denatured conditions.>50% ribonuclease B (10 µg) is deglycosylated by 10 ng of rFmPNGase F within 30 minutes, as measured under the described conditions.
Molecular Mass : Recombinant F. meningosepticum PNGF, Met&His-tagged has a calculated MW of 36 kDa. In SDS-PAGE migrates as 33-35 kDa, reducing conditions.
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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