"PNGF" Related Products

Recombinant F. meningosepticum PNGF, Met&His-tagged

Cat.No.: PNGF-200F
Product Overview: Recombinant F. meningosepticum PNGF (Accession # AAA85323) Ala41-Asn354, fused with N-terminal Met and 6-His tag, was produced in E. coli-derived.
Source: E. coli
Species: F. meningosepticum
Tag: &His
Predicted N Terminal: Met
Form: Supplied as a 0.2 µm filtered solution in Tris and NaCl.
Bio-activity: Measured by its ability to deglycosylate ribonuclease B under denatured conditions.>50% ribonuclease B (10 µg) is deglycosylated by 10 ng of rFmPNGase F within 30 minutes, as measured under the described conditions.
Molecular Mass: Recombinant F. meningosepticum PNGF, Met&His-tagged has a calculated MW of 36 kDa. In SDS-PAGE migrates as 33-35 kDa, reducing conditions.
Purity: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

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