Active Recombinant Mouse Dkkl1, His-tagged

• Cat.No.: Dkkl1-277M
• Product Overview: Recombinant Mouse Dkkl1 (Accession # AAF02679) Leu21-Leu230, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: His
• Protein Length: 21-230 a.a.
• Predicted N Terminal: Leu21
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells.The ED50 for this effect is typically 2 - 6 µg/mL.
• Molecular Mass: Recombinant Mouse Dkkl1, His-tagged has a calculated MW of 26.8 kDa. In SDS-PAGE migrates as 40 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Dkkl1 dickkopf-like 1 [ Mus musculus ]
• Official Symbol: Dkkl1
• Synonyms: DKKL1; dickkopf-like 1; dickkopf-like protein 1; soggy 1; protein soggy-1; SGY; SGY1; mSgy; SGY-1; Soggy; AI414495
• Gene ID: 50722
• mRNA Refseq: NM_015789
• Protein Refseq: NP_056604