||Recombinant human BMF, fused to T7-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography, 15.6 kDa (144aa)
||Bcl2 modifying factor, also known as BMF belongs to the Bcl2 protein family of apoptosis mediators. Bmf is constitutively expressed in many tissues. This protein contains a single Bcl2 homology domain 3 (BH3), and has been shown to bind Bcl2 proteins and function as an apoptotic activator. Also, BMF is a key molecule for histone deacetylase (HDAC) inhibitors which alters the balance between acetylation and deacetylation, significantly increasing histone acetylation, while strongly inducing apoptosis in a variety of cancer cell types.
||> 90% by SDS - PAGE
|Sequences of amino acids:
||MASMTGGQQM GRGSHMEPSQ CVEELEDDVF QPEDGEPVTQ PGSLLSADLF AQSLLDCPLS RLQLFPLTHC CGPGLRPTSQ EDKATQTLSP ASPSQGVMLP CGVTEEPQRL FYAPAEPKSC VVADPPLPAQ PCFEWRREQE RGRP
||1 mg/ml (determined by Bradford assay)
||Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing, 1mM DTT, 10% glycerol
||< 1.0 EU per 1 μg of protein (determined by LAL method )
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.