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Agarose Fast Flow

Cat.No. : Agarose-003A
Product Overview : Agarose Fast Flow gel filtration medium is based on cross-linked agarose matrix, which gives good physical stability and chromatographic qualities. It is a gel filtration medium developed for industrial processing at high flow rates and moderate pressures.
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Cat.no : Agarose-003A
Description : The matrix was developed specifically to meet the high throughput demands of industrial process separations. The rigidity permits high flow rates, which in turn gives good resolution in a minimum of time. Agarose 4 Fast Flow matrices enable the rapid processing of large volumes making these media an excellent choice for use at process-scale.Agarose Fast Flow gel filtration media are easy to work with, and tolerate working conditions of temperature, pH and chemical agents, including cleaning-in-place (CIP) reagents, typically used in biopharmaceutical production processes. The bed should be washed with at least 3 bed volumes of buffer to remove the preservative.
Matrix : 4% or 6% cross-linked agarose
Average particle size : 90 µm
Chemical stability : 2 M NaOH, 70% ethanol, 30% isopropanol, 30% acetonitrile, 1% SDS, 2% Asepto, 6 M guanidine-hydrochloride, 8 M urea [40°C for 7 Days in]
pH working range : 2-12
pH CIP range : 2-14
Autoclavable : 121°C for 20 min in H2O
Storage : 3 to 40°C, 20% Ethanol.

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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (21)

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Are there different types of agarose available for gel electrophoresis? 09/19/2022

Yes, agarose can vary in its gel strength and pore size, affecting its ability to separate molecules of different sizes.

Can agarose separate proteins? 05/13/2022

Agarose gel electrophoresis is primarily used for nucleic acids (DNA, RNA) separation. Proteins are often separated using polyacrylamide gels (SDS-PAGE).

What is the purpose of a buffer in agarose gel electrophoresis? 07/19/2021

The buffer maintains a stable pH and provides ions to carry the electric current, facilitating the movement of molecules through the gel.

Is agarose gel electrophoresis quantitative? 06/24/2021

Agarose gel electrophoresis provides qualitative information about molecular size, but not precise quantification of amounts.

What is the significance of agarose concentration in the gel? 05/15/2021

Agarose concentration determines the size range of molecules that can be separated; lower concentration gels are better for larger molecules, and vice versa.

Can agarose gels be used to separate proteins based on their charge? 06/22/2020

No, agarose gels do not provide a suitable matrix for separating proteins based on charge; that's better achieved with techniques like isoelectric focusing.

Is agarose gel electrophoresis suitable for high-resolution separation? 01/23/2020

No, agarose gels provide relatively low resolution compared to techniques like capillary electrophoresis or high-resolution polyacrylamide gels.

What is the purpose of the loading dye in agarose gel electrophoresis? 12/11/2019

Loading dye helps visualize sample loading and migration during electrophoresis. It adds density to the samples, making them sink into the wells, and provides color for tracking progress.

Can agarose gels be used for preparative purposes? 09/11/2019

Yes, agarose gels can be used for isolating DNA fragments of interest by cutting out the desired band from the gel.

What is the purpose of the comb used when casting agarose gels? 08/31/2019

The comb creates wells in the gel where samples are loaded. After the gel solidifies, the comb is removed, leaving empty wells for sample loading.

How is agarose gel electrophoresis performed? 05/30/2018

Agarose gel electrophoresis involves placing DNA, RNA, or proteins in wells on an agarose gel, applying an electric field, and watching them migrate through the gel matrix based on their size.

Can agarose gels be used to separate protein complexes? 11/23/2017

Agarose gels are not typically used for separating protein complexes; techniques like native PAGE or blue native PAGE are better suited for this purpose.

How are protein samples prepared for agarose gel electrophoresis? 08/24/2017

Protein samples are often mixed with a loading buffer that provides color and density for loading, and sometimes a reducing agent to break disulfide bonds.

Can agarose gels separate RNA molecules? 05/31/2017

Yes, agarose gel electrophoresis can also separate RNA molecules based on their size, similar to how it separates DNA.

What is the purpose of a DNA ladder in gel electrophoresis? 05/02/2017

A DNA ladder consists of DNA fragments of known sizes. It helps estimate the size of unknown DNA fragments by comparing their migration distances to those of the ladder's fragments.

Can proteins be denatured and separated using agarose gels? 04/15/2017

While agarose gels are not ideal for protein separation, native protein conformation can be preserved, and small proteins may be separated to some extent.

What is agarose gel's function in this process? 03/12/2017

Agarose gel provides a porous medium through which biomolecules can move, separating them by size as smaller molecules migrate faster through the gel.

Are there alternatives to agarose gels for protein separation? 02/12/2017

Yes, polyacrylamide gels (SDS-PAGE) are commonly used for protein separation due to their higher resolution and ability to denature proteins.

What is agarose? 10/05/2016

Agarose is a polysaccharide derived from seaweed, commonly used in gel electrophoresis for separating DNA, RNA, and proteins.

How does the voltage applied during electrophoresis affect agarose gel separation? 09/23/2016

Higher voltages can lead to faster migration but may cause heat buildup and distortion. Optimal voltage depends on the gel's thickness and size of molecules being separated.

How is DNA visualized in an agarose gel? 04/14/2016

DNA is often stained with fluorescent dyes, like ethidium bromide or SYBR Green, which bind to DNA and emit visible light under UV illumination.

Customer Reviews (5)

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Reviews
05/31/2021

     Replicating outcomes across diverse cell lines underscored the product’s consistent behavior.

    03/04/2021

      Our data indicates that Agarose promotes neurite outgrowth effectively.

      05/31/2018

        The product’s insights justified its cost, representing a valuable investment in our research.

        03/01/2017

          It’s reliability was evident in consistent protein-protein interactions.

          04/18/2016

            It's purity enhanced the accuracy of mass spectrometry results.

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