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Recombinant Mouse Nphs1, His-tagged

Cat.No.: Nphs1-16M
Product Overview: Recombinant Mouse Nphs1(Gln37-Thr1049) fussed with 6-His tag at C-terminal was expressed in NS0 cell.
Description: Nephrin is a 185 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily. Mature mouse Nephrin consists of a 1042 amino acid (aa) extracellular domain (ECD) with eight Ig­like C2­set domains and one fibronectin type III domain, a 22 aa transmembrane segment, and a 156 aa cytoplasmic tail. Within the ECD, mouse Nephrin shares 84% and 95% aa sequence identity with human and rat Nephrin, respectively. Usage of the alternate exon 1B results in a distinct N­terminal sequence that lacks a clearly defined signal peptide cleavage site. Nephrin is expressed primarily on podocytes in the renal glomerulus and to a lesser extent in the brain and pancreas. The 1B isoform is not expressed in the kidney. Nephrin localizes to intercellular junctions between podocyte foot processes where it functions as a homophilic adhesion molecule. Nephrin is required for formation and maintenance of the slit diaphragm between these processes. It associates with Neph1, podicin, P­cadherin, and multiple scaffolding proteins which couple it to the actin cytoskeleton. Nephrin expression is required for the anti­apoptotic effect of VEGF on podocytes as well as for the ability of podocytes to upregulate Glut1 and Glut4 glucose transporters in response to insulin. Nephrin downregulation contributes to diabetic nephropathy, and Nephrin mutations underlie the lethal congenital nephritic syndrome NPHS1.
Source: NS0 cell
Species: Mouse
Tag: His
Predicted N Terminal: Gln37 predicted
Form: Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of MS-1 mouse pancreatic islet endothelial cells.When 5 x 10^4 cells/well are added to rmNephrin coated plates (10 µg/mL, 100 µL/well), approximately 60 - 80% will adhere after 90 minutes at 37° C.
Molecular Mass: Predicted Molecular Mass:110 kDaSDS-PAGE:130-145 kDa, reducing conditions
Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Stability: Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied;1 month, 2 to 8 centigrade under sterile conditions after reconstitution;3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution: Reconstitute at 100 µg/mL in sterile PBS.
Gene Name: Nphs1 nephrosis 1, nephrin [ Mus musculus ]
Official Symbol: Nphs1
Synonyms: nephrin; nephrin 1; nephrosis 1 homolog, nephrin; renal glomerulus-specific cell adhesion receptor
Gene ID: 54631
mRNA Refseq: NM_019459
Protein Refseq: NP_062332
UniProt ID: Q9QZS7
Chromosome Location: 7; 7 A3
Pathway: Cell-Cell communication, organism-specific biosystem; Nephrin interactions, organism-specific biosystem; PodNet: protein-protein interactions in the podocyte, organism-specific biosystem
Function: alpha-actinin binding; myosin binding; protein binding

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