Active Recombinant Human CILP Protein, His-tagged

• Cat.No.: CILP-538H
• Product Overview: Recombinant Human CILP (Accession # NP_003604) Met1-Arg720, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: Met1-Arg720
• Predicted N Terminal: Arg22
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.The ED50 for this effect is typically 0.5 - 2.0 µg/mL. Optimal dilutions should be determined by each laboratory for each application.Also measured by its ability to bind Recombinant Human TGF-beta 1.
• Molecular Mass: Recombinant Human CILP, His-tagged has a calculated MW of 78.8 kDa. In SDS-PAGE migrates as 85-95 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 50 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: CILP cartilage intermediate layer protein, nucleotide pyrophosphohydrolase [ Homo sapiens ]
• Official Symbol: CILP
• Synonyms: CILP; cartilage intermediate layer protein, nucleotide pyrophosphohydrolase; cartilage intermediate layer protein 1; HsT18872; cartilage intermediate-layer protein; cartilage intermediate layer protein 1 C1; cartilage intermediate layer protein 1 C2; CILP-1
• Gene ID: 8483
• mRNA Refseq: NM_003613
• Protein Refseq: NP_003604
• MIM: 603489
• UniProt ID: O75339
• Chromosome Location: 15q22