Recombinant Human CILP Protein, His-tagged CILP-538H

Recombinant Human CILP Protein, His-tagged

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Recombinant Human CILP Protein, His-tagged

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Cat.No. : CILP-538H
Product Overview : Recombinant Human CILP (Accession # NP_003604) Met1-Arg720, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source : NSO Cells
Species : Human
Tag : His
Predicted N Terminal : Arg22
Form : Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity : Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.The ED50 for this effect is typically 0.5 - 2.0 µg/mL. Optimal dilutions should be determined by each laboratory for each application.Also measured by its ability to bind Recombinant Human TGF-beta 1.
Molecular Mass : Recombinant Human CILP, His-tagged has a calculated MW of 78.8 kDa. In SDS-PAGE migrates as 85-95 kDa, reducing conditions.
Protein length : Met1-Arg720
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 50 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name : CILP cartilage intermediate layer protein, nucleotide pyrophosphohydrolase [ Homo sapiens ]
Official Symbol : CILP
Synonyms : CILP; cartilage intermediate layer protein, nucleotide pyrophosphohydrolase; cartilage intermediate layer protein 1; HsT18872; cartilage intermediate-layer protein; cartilage intermediate layer protein 1 C1; cartilage intermediate layer protein 1 C2; CILP-1;
Gene ID : 8483
mRNA Refseq : NM_003613
Protein Refseq : NP_003604
MIM : 603489
UniProt ID : O75339
Chromosome Location : 15q22

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