||Recombinant TEV TEVgp1 (Accession # NP_062908), fused with , was produced in E. coli.
|Predicted N Terminal:
||Supplied as a 0.2 µm filtered solution in Glycerol, Tris, NaCl, EDTA and DTT.
||Measured by its ability to cleave a fusion protein containing the recognition sequence Glu-Asn-Leu-Tyr-Phe-Gln, with the cleavage point after Gln.TEV Protease cleaves =50% of the control substrate, as measured under the described conditions. . It is recommended that the cleavage for each fusion protein be optimized by varying the amount of Recombinant Viral TEV Protease, reaction time, or incubation temperature.
||Recombinant TEV TEVgp1 has a calculated MW of 28.5 kDa. In SDS-PAGE migrates as 28 kDa, reducing conditions.
||>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
||Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).